Nephroprotective of Anthocyanin Pigments Extract From Red Cabbage (Brassica oleracea L. Var. Capitata F. Rugba) Against Gentamicin-Captopril-Induced Nephrotoxicity in Rats (ARTIKEL)

Herlina, Herlina (2023) Nephroprotective of Anthocyanin Pigments Extract From Red Cabbage (Brassica oleracea L. Var. Capitata F. Rugba) Against Gentamicin-Captopril-Induced Nephrotoxicity in Rats (ARTIKEL). Asian Journal of Pharmaceutical and Clinical Research, 11 (4). pp. 432-436. ISSN 2455-3891

Text AJPCR_11_4_2018 SHAUM S.pdf Download (910kB)

Abstract

ABSTRACT Objective: Red cabbage (Brassica oleracea L. var. Capitata f. rubra) has a fairly high anthocyanin content and is a source of powerful antioxidants. This study is basic for the development of a nutraceutical, which has nephroprotective activity. Methods: Red cabbage was extracted using ethanol and water with a mixture of citric acid with a variation of 1, 2, and 3%. The total anthocyanin was determined with pH-differential method. The Wistar strain male rats were divided into five groups. Group 1 as a negative control, Group 2 as a positive control treated with Vitamin E dose of 400 mg/kg BW day. Groups 3–5 were treated with three different extract dose of 100, 200, and 400 mg/kg BW. Gentamicin was given intraperitoneally and captopril orally for 3 days. Extracts and Vitamin E were administrated orally for 15 days after induction of gentamicin-captopril. Nephroprotective activity was determined by measuring the levels of serum creatinine, blood ureum, and macroscopic kidney. Results: The combination of 96% ethanol and citric acid 3% showed the percent of free radical 2,2-diphenyl-1-picrylhydrazyl arrest of 75.23% and contained 53.49 ± 5.01 mg/L of total anthocyanin. The anthocyanin pigment from red cabbage extract can decrease the levels of creatinine and ureum, which dose of 100 mg/kg BW showed the highest value of 48.72%. There were differences in the macroscopic morphology in the rat kidney. Conclusion: Based on the results, we concluded that ethanol with 3% citric acid produced higher anthocyanin and showed nephroprotective activity. Keywords: Anthocyanin, Brassica oleracea L., Captopril, Gentamicin, Nephroprotective, Creatinine.

Item Type:	Article
Subjects:	R Medicine > RS Pharmacy and materia medica > RS160-167 Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Divisions:	08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User:	Herlina Herlina
Date Deposited:	07 Jun 2023 03:21
Last Modified:	07 Jun 2023 03:21
URI:	http://repository.unsri.ac.id/id/eprint/107595

Actions (login required)

View Item