Antihyperlipidemia Activity of Ethyl Acetate Fraction from Melinjo (Gnetum gnemon Linn.) Leaf in White Male Wistar Rats Induced by Propylthiouracil (ARTIKEL)

Herlina, Herlina (2023) Antihyperlipidemia Activity of Ethyl Acetate Fraction from Melinjo (Gnetum gnemon Linn.) Leaf in White Male Wistar Rats Induced by Propylthiouracil (ARTIKEL). Atlantis Press, 40. pp. 110-119. ISSN 2468-5739(printed ISSN) 978-94-6252-978-6(e-ISSN)

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Abstract

The antihyperlipidemia activity of ethyl acetate fraction from Melinjo leaf (Gnetum gnemon L.) against white male wistar rats induced by propylthiouracil has been carried out. Wistar male rats were divided into six groups, which are a normal, a positive control, a negative control, and the other three groups with a dose treatment of 10 mg/kg BW, 30 mg/kg BW, and 50 mg/kg BW. Experimental hyperlipidemic rats were induced by daily intake 0.01% PTU and high-fat for the duration of 2 weeks except the normal group was only given standard feed. Hyperlipidemic rats group were orally with three doses (10, 30, dan 50 mg/kg BW) of the ethyl acetate fraction for 2-weeks onward. The cholesterol and triglycerides levels were measured by using enzymatic method of CHOD-PAP and GPO-PAP. HDL levels were measured by using Cholesterol HDL Analysis Kit, and LDL levels were calculated by using indirect calculation. Decreased triglycerides levels at dose of 10 mg/kg BW and increased HDL levels at dose 30, 50 mg/kg BW were not significantly different with simvastatin (p>0.05), while decreased cholesterol and LDL levels at dose 10, 30, 50 mg/kg BW were significantly different with simvastatin (p <0,05). Based on the relationship between the percentage reduction in cholesterol, triglycerides, LDL and increased of HDL level, the effective ED50 of ethyl acetate fraction of Melinjo leaf is 34,74 mg/kgBW. Histopatological analyses of liver’s rat showed that dose of 50 mg/kg BW was the same as simvastatin in its the percentage of necrosis and the percentage of fat degeneration of 20%. These results indicate that the ethyl acetate fraction of Melinjo leaf has an antihyperlipidemia, and there is a significant difference compared to the negative control (p <0,05).

Item Type:	Article
Subjects:	R Medicine > RS Pharmacy and materia medica > RS160-167 Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Divisions:	08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User:	Herlina Herlina
Date Deposited:	09 Jun 2023 12:49
Last Modified:	09 Jun 2023 12:49
URI:	http://repository.unsri.ac.id/id/eprint/107734

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