KINETIKA HIDROLISIS ENZIMATIK SELULOSA BATANG PISANG YANG DIBERI PRA-PERLAKUAN ALKALINE MICROWAVE-ASSISTED

SAMARA, FARAS SASKIA and Novia, Novia and Melwita, Melwita (2023) KINETIKA HIDROLISIS ENZIMATIK SELULOSA BATANG PISANG YANG DIBERI PRA-PERLAKUAN ALKALINE MICROWAVE-ASSISTED. Masters thesis, Sriwijaya University.

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Abstract

The banana stem waste holds immense promise as a readily available and abundant source of lignocellulosic biomass, making it a compelling alternative for biofuel and biochemical applications. Therefore, this study focuses on investigating the impact of both time and substrate loading on the enzymatic hydrolysis of banana stem cellulose that has undergone alkaline microwave-assisted pre-treatment. The pre-treatment method involves subjecting the biomass to 5% KOH for 30 min, followed by microwave exposure at 300 W for 5 min, a process aimed at enhancing the accessibility of cellulose. Enzymatic hydrolysis experiments were carried out utilizing cellulase enzymes derived from Aspergillus niger, with variations in hydrolysis times (ranging from 10 to 35 h) and enzyme-to-substrate ratios (ranging from 1:1 to 1:10). The results of this investigation revealed a substantial improvement in hydrolysis efficiency, owing to the synergistic effects of alkaline microwave-assisted pre-treatment, signifying enhanced cellulose accessibility. Notably, the highest concentration of reducing sugars (1,3 mg mL−1) was achieved at a substrate-to-enzyme ratio of 1:1 and a hydrolysis duration of 35 h. These findings provide valuable insights into the conversion of lignocellulosic biomass, emphasizing the potential of integrated pre-treatment strategies for sustainable biorefinery applications. This research contributes to advancing our understanding of lignocellulosic biomass utilization, offering a promising avenue for biofuel and biochemical production from banana stem waste.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Pra-perlakuan alkaline microwave-assisted, Batang pisang, Hidrolisis enzimatik, Gula pereduksi total
Subjects:	T Technology > TP Chemical technology > TP155-156 Chemical engineering T Technology > TP Chemical technology > TP248.13-248.65 Biotechnology
Divisions:	03-Faculty of Engineering > 24101-Chemical Engineering (S2)
Depositing User:	Faras Saskia Samara
Date Deposited:	19 Dec 2023 04:52
Last Modified:	19 Dec 2023 04:52
URI:	http://repository.unsri.ac.id/id/eprint/135322

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