UJI BIO-INSEKTISIDA BERBAHAN AKTIF Bacillus thuringiensis PADA BERBAGAI MEDIA LIMBAH PADAT TERHADAP MORTALITAS LARVA Oryctes rhinoceros (COLEOPTERA: SCARABAEIDAE) PADA SUHU 30-35°C

FEBRIYANTI, NABILA and Pujiastuti, Yulia (2023) UJI BIO-INSEKTISIDA BERBAHAN AKTIF Bacillus thuringiensis PADA BERBAGAI MEDIA LIMBAH PADAT TERHADAP MORTALITAS LARVA Oryctes rhinoceros (COLEOPTERA: SCARABAEIDAE) PADA SUHU 30-35°C. Undergraduate thesis, Sriwijaya University.

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Abstract

The horned beetle (Oryctes rhinoceros) is a major pest of oil palm, due to its attack can affect the yield and productivity of plant. Control of O. rhinoceros pests commonly carried out by farmers and oil palm plantation companies is by using synthetic insecticides. However, the continuous use of synthetic insecticide can cause negative impacts such as resistance, pest recurrence, killing of natural enemy, and environmental pollution. Thus, reducing the use of synthetic insecticides in oil palm plantations requires other safe and environmentally friendly control methods, including using bio-insecticides made from Bacillus thuringiensis is an alternative that has been used to control various pest species. Therefore, this research aims to determine the effectiveness of using a mixture of solid waste media in influencing the development of B.thuringiensis and the level of toxicity to the mortality of O. rhinoceros larvae at a static temperature of 30-35°C. Observations indicated that larvae mortality was significantly different between treatments. This research was conducted in the Integrated Biological Control Laboratory, Department Plant Protection, Faculty Agriculture. The device used was an incubator at a static temperature of 30-35°C. This research used a Completed Randomized Design (CRD) consisting of 6 treatments and 4 replication, as a comparison water control and commercial bioinsecticides were used. The test insects used in this research were the 3rd instar O. rhinoceros larvae. In each replication in each treatment, there were six O. rhinoceros larvae used as test insects. The application was carried out in plastic containers placed in an incubator, each of which was given a treatment, namely P1 (rice bran+oil palm cake) (2:1), P2 (rice bran+tofu dregs) (2:1), P3 (rice bran+peanut cake) (2:1), P4 (rice bran+corn huks) (2:1), P5 (commercial bio-insecticide), P6 (water control). The highest mortality was found in treatment P1 with the fastest mortality percentage reaching 50% at 6.24 days after application while the lowest mortality in treatment P4 reached 50% at 11.9 days after application. The symptoms caused by O. rhinoceros larvae that have been infected with B. thuringiensis bacteria were the larvae's body become soft, change colour, move lazily and the dead larvae will turn brown to black, discharge that has an unpleasant odour. The use of a mixture of solid waste media in influencing the development of B. thuringiensis, namely oil palm cake which can be used as a nitrogen source with the highest colony density at 72 hours after incubation of 3.58x1010 cfu/g, and the highest mortality occurred in the rice bran+oil palm cake treatment with 50% mortality rate occurred at 6.24 days after application.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Incubator, Biological Control, Test Insect
Subjects:	S Agriculture > SB Plant culture > SB599-990.5 Pests and diseases S Agriculture > SB Plant culture > SB950-990.5 Pest control and treatment of diseases. Plant protection S Agriculture > SB Plant culture > SB974-978 Organic plant protection. Biological control
Divisions:	05-Faculty of Agriculture > 54295-Plant Protection (S1)
Depositing User:	Nabila Febriyanti
Date Deposited:	05 Jan 2024 08:24
Last Modified:	05 Jan 2024 08:24
URI:	http://repository.unsri.ac.id/id/eprint/137579

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