DNA BARCODE IKAN TILAN (Mastacembelus sp.) ASAL SUNGAI MUSI DAN SUNGAI ENIM BERDASARKAN GEN SITOKROM C OKSIDASE SUBUNIT I (COI)

NINGSIH, REZKY AMANDA and Syaifudin, Mochamad and Mukti, Retno Cahya (2023) DNA BARCODE IKAN TILAN (Mastacembelus sp.) ASAL SUNGAI MUSI DAN SUNGAI ENIM BERDASARKAN GEN SITOKROM C OKSIDASE SUBUNIT I (COI). Undergraduate thesis, Sriwijaya University.

	Text RAMA_54243_05051281924020.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (9MB) | Request a copy
	Text RAMA_54243_05051281924020_TURNITIN.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (2MB) | Request a copy
	Text RAMA_54243_05051281924020_0003037602_0027108901_01_front_ref.pdf - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (3MB)
	Text RAMA_54243_05051281924020_0003037602_0027108901_02.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (882kB) | Request a copy
	Text RAMA_54243_05051281924020_0003037602_0027108901_03.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy
	Text RAMA_54243_05051281924020_0003037602_0027108901_04.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy
	Text RAMA_54243_05051281924020_0003037602_0027108901_05.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (99kB) | Request a copy
	Text RAMA_54243_05051281924020_0003037602_0027108901_06_ref.pdf - Bibliography Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy
	Text RAMA_54243_05051281924020_0003037602_0027108901_07_lamp.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy

Abstract

Spiny eel (Mastacembelus sp.) is a freshwater fish that is distributed on the islands of Java, Sumatera and Kalimantan. Mastacembelus erythrotaenia has been found in the Musi River and Mastacembelus unicolor has been found in the Enim River, the two species have different colors and patterns on their bodies. Therefore, it is necessary to study the genetic characteristics of these two species. This research aims to obtain the quantity and quality of spiny eel DNA, determine the COI gene sequence of spiny eel mitochondrial DNA and the percentage of similarities between spiny eel and other fish in GenBank data, as well as determine the water quality in the spiny eel habitat. This research was carried out in February to August 2023. The methods used in barcode were DNA extraction, DNA amplification, electrophoresis and sequencing of the COI gene region. The PCR product was obtained from the amplification with an annealing temperature of 49.2°C for 45 seconds in 31 cycles. The results showed that the DNA quantity value of spiny eel from the Musi and Enim Rivers ranged from 55.1-131.9 ng μL-1, the absorption ratio of A260/A280 from 1.64-1.98 and A260/230 from 1.60-2.73. The sequence of PCR products produced nucleotide lengths from 207-388 bp. The percentage of similarities between spiny eel and other fish in GenBank data cannot be determined. Transparency values ranged from 21.25-42 cm, dissolved oxygen (DO) from 3.52-7.66 mg L-1, current velocity from 0.14-0.47 m s-1, alkalinity from 20-40 mg L-1, pH from 6.57-7.6, temperature from 25.2-28.9°C, total dissolved solid (TDS) from 11-15 mg L-1, ammonia from 0.20-0.59 mg L-1.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	COI gene, DNA barcode, Enim River, Musi River, spiny eel
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture
Depositing User:	Rezky Amanda Ningsih
Date Deposited:	09 Jan 2024 04:22
Last Modified:	09 Jan 2024 04:22
URI:	http://repository.unsri.ac.id/id/eprint/137752

Actions (login required)

View Item