Amebocyte lysate Asian horseshoe crab for bacterial endotoxin test from the estuary waters of Banyuasin, South Sumatra

Priyanto, Langgeng (2023) Amebocyte lysate Asian horseshoe crab for bacterial endotoxin test from the estuary waters of Banyuasin, South Sumatra. In: SRIWIJAYA INTERNATIONAL CONFERENCE ON BASIC AND APPLIED SCIENCES 2021, 2 November 2021, Palembang.

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Abstract

Abstract. Horseshoe crab blood contains amebocyte lysate which is very important in the process of detecting bacterial endotoxins. When endotoxin enters the bloodstream, the inflammatory response of our immune system causes damage to blood vessels. The purpose of this study was to determine the ability of Asian Horseshoe Crab (AHC) amebocyte lysate from the Estuary waters of Banyuasin, South Sumatra to detect endotoxin bacteria. A sampling of horseshoe crabs was carried out in March 2021. The 28 individual horseshoe crabs were identified using morphometrics and 10 ml of blood was taken from each individual. Afterward, the horseshoe crabs were released back into their habitat. The bacterial endotoxin test (BET) is based on the qualitative method (Gel Clot) and quantitative method (Chromogenic). The bacterial endotoxin concentrations used were 1 EU/ml, 0.5 EU/ml, 0.25 EU/ml and 0.125 EU/ml. The identification species showed 2 species of AHC, namely Carcinoscorpius rotundicauda and Tachypleus gigas. Out of 28 individuals for amebocyte lysate assay resulted 4 individuals were able to detect the presence of endotoxin bacteria using the Gel Clot method. It is characterized by the presence of a weak coagulant with a maximum endurance of 12 seconds of incubation. The Chromogenic method can only detect one individual of T. gigas with a very strong correlation (r = 0.905) within 60 minutes of incubation. The results showed that the amebocyte lysate of AHC from the Estuary waters of Banyuasin, South Sumatra could detect the presence of bacterial endotoxin. Keywords: Amebocyte lysate, Bacterial Endotoxin, Chromogenic, Gel Clot, South Sumatra

Item Type:	Conference or Workshop Item (Paper)
Subjects:	S Agriculture > SB Plant culture > SB974-978 Organic plant protection. Biological control
Divisions:	05-Faculty of Agriculture > 54231-Animal Husbandry (Animal Science) (S1)
Depositing User:	Langgeng Priyanto
Date Deposited:	25 Jan 2024 03:26
Last Modified:	25 Jan 2024 03:26
URI:	http://repository.unsri.ac.id/id/eprint/138399

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