UJI POTENSI IMUNOMODULATOR PADA MENCIT (MUS MUSCULUS) DAN ANTI PROLIFERASI SACCHAROMYCES CEREVISIAE DARI EKSTRAK METANOL DAUN SUNGKAI (PERONEMA CANESCENS JACK)

KAMAL, IRENA FRANCINA KELLY and Hermansyah, Hermansyah and Desnelli, Desnelli (2024) UJI POTENSI IMUNOMODULATOR PADA MENCIT (MUS MUSCULUS) DAN ANTI PROLIFERASI SACCHAROMYCES CEREVISIAE DARI EKSTRAK METANOL DAUN SUNGKAI (PERONEMA CANESCENS JACK). Undergraduate thesis, Sriwijaya University.

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Abstract

Sungkai (Peronema canescens Jack.) is one of woody plants which is usually found in the Southern part of Sumatra which is often used as an herbal plant by people for flu and fever. This research was aimed to know immunomodulator potency of Sungkai in mice (Mus muculus) and effect extract to proliferation of Saccharomyces cerevisiae yeast and leukocytes. Yeast proliferation is associated with inflammatory activity while leukocytes assay is associated closely with immunostimulatory activity. Sungkai leaf was macerated using methanol then evaporated and tested for its phytochemistry profile and thin layer chromatography. Several compound groups were detected in phytochemistry profiling, such as terpene, steroid, alkaloid, saponin, and flavone. For the leukocytes assay of the extract, mice were used. There were several variables, positive control (Stimuno), negative control (CMC-Na), 5% concentration, 10% concentration, and 15% concentration. Mice leukocytes were taken from tail biopsy and counted under haemocytometer and microscope. 15% concentration extract results in the highest content of leukocytes found in the mice tail which was 9560 units. This indicated that sungkai extract has potency as immunomodulator as was tested with p<0.05. This extract was then tested for its proliferation activity of S. cerevisiae yeast growth by adding some extract of various concentrations, 5%, 10%, 15%, and 20%, to yeast culture broth. Based on optical density observation using spectrophotometer UV-Vis OD600 show that there was no significant difference (p>0.05) cell growth among in the absence and in the presence of various concentrations of extract. This indicated that sungkai has no effect for the proliferation of S.cerevisiae cell.

Item Type:	Thesis (Undergraduate)
Subjects:	Q Science > QD Chemistry > QD415-436 Biochemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Irena Francina Kelly Kamal
Date Deposited:	23 Jan 2024 02:30
Last Modified:	23 Jan 2024 02:30
URI:	http://repository.unsri.ac.id/id/eprint/139256

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