BARCODING DNA ISOLAT BAKTERI BERPOTENSI SEBAGAI PROBIOTIK ASAL SEDIMEN RAWA

SUAHADA, JANUAR AHLAN and Wijayanti, Marini and Jubaedah, Dade (2018) BARCODING DNA ISOLAT BAKTERI BERPOTENSI SEBAGAI PROBIOTIK ASAL SEDIMEN RAWA. Undergraduate thesis, Sriwijaya University.

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Abstract

Bacteria derived from swamp sediments and aquaculture ponds potential to be probiotics. The research aims to determine the sequence of 16S rRNA gene of isolate of probiotics candidate bacteria from sediment of swamp and pangasius pond, to determine the phylogenetic tree between the bacterial species from isolates and GeneBank data central for their potentially as probiotic. The samples of bacteria resulted from pure isolation selected from the sediment of pond cultivation and swamp waters at Lebung Karangan Reservation, Ogan Ilir Regency, Indralaya, South Sumatra. This study was started from cultivating bacteria, extracting DNA of bacteria, amplificating 16S rRNA genes by PCR, running electrophoresis, and sequencing the amplicon for determining DNA barcodes of bacteria from sediment of swamp and rearing pond. Results of 16S sRNA gene sequencing were obtained nucleotide length 1324bp (KA Isolate), 1328bp (RA Isolate), 1324bp (KE Isolate), and 1342bp (RE Isolate). The result of BLAST analysis showed that KA isolate has the highest similarity 97% with Streptomyces sp. Hjorring101 from Denmark and RA isolate had the highest similarity 98% with Streptomyces sp. BD99 from Pakistan. KE isolate had the highest similarity 99% with Bacillus subtilis CESi5 from Japanese and RE isolate had the highest similarity 93% with Bacillus sp.2bFR from Manado. All of isolates were bacteria potentially as aquaculture probiotics. Water quality in cultivation medium were temperature 29,3-29,90C, pH 6,0-8,2, dissolved oxygen 3,3-5,0 mgL-1. All of isolates were bacteria potentially as aquaculture probiotics.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Bacteria, isolate, 16S rRNA gene, sequencing, probiotic.
Subjects:	S Agriculture > S Agriculture (General) > S1-(972) Agriculture (General) S Agriculture > S Agriculture (General) > S588.4-589.6 Agricultural physics. Including radioisotopes in agriculture
Divisions:	05-Faculty of Agriculture > 54243-Aquaculture (S1)
Depositing User:	Mr. Muhammad Irwan
Date Deposited:	31 Oct 2019 05:02
Last Modified:	31 Oct 2019 05:02
URI:	http://repository.unsri.ac.id/id/eprint/14072

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