PEMERIKSAAN SISTEMATIK MORFOLOGI, METAPHASE KARYOTYPES, CROSSING EXPERIMENT, DAN MOLEKULER NYAMUK VEKTOR MALARIA ANOPHELES VAGUS DAN ANOPHELES LIMOSUS

DALILAH, DALILAH and Anwar, Chairil and Syafruddin, Syafruddin and Saleh, Irsan (2024) PEMERIKSAAN SISTEMATIK MORFOLOGI, METAPHASE KARYOTYPES, CROSSING EXPERIMENT, DAN MOLEKULER NYAMUK VEKTOR MALARIA ANOPHELES VAGUS DAN ANOPHELES LIMOSUS. Doctoral thesis, SRIWIJAYA UNIVERSITY.

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Abstract

Background. Literature review studies that have been conducted describe that An. vagus is a monophyletic species, and together with An. limosus are conspecifics. This study aims to examine the diversity of field mosquitoes in South Sumatra and Lampung, re-examine the morphology, molecular and phylogenetics of An. vagus and An. limosus mosquitoes from field samples and from stored specimen from , South Sumatra, Lampung, East Java, West Sumatra and South Sulawesi. In addition, metaphase karyotyping and crossmating experiments were conducted. All to confirm and conclude whether An. vagus and An. limosus mosquitoes are monophyletic or species complex. Method. This study was a descriptive field survey and laboratory analysis with a molecular study approach. Adult mosquitoes were captured using Human Landing Collection (HLC) Resting Collection (RC) and Cattle Bait Collection (CBC) techniques. Molecular examination using nDNA ITS2, mtDNA COI and mtDNA ND6 markers specifically designed for this study. Results. The diversity index of Anopheles in the three villages was 0.66; 0.82; and 0.35, respectively. Morphological observations found An. vagus characterized by the length of the pale band on the palpi three times the length of the dark band below, and there are four pale bands on the costa. Karyotyping of metaphase was not successful due to the unsuccessful colonization process of the larvae. Anopheles limosus mosquitoes were not found in the study area, so crossmating experiments could not be conducted. Sequencing results of An. vagus mosquitoes showed similarity of mtDNA COI, nDNA ITS2 and mtDNA ND6 respectively: 98.26-100%; 99-100%; and 100%. Phylogenetic results found two groups of An. vagus with mtDNA COI markers (distance 0.00-0.02) and one group on nDNA ITS2 and mtDNA ND6 markers (distance 0.00-0.01). Conclusion. The diversity of Anopheles genus mosquitoes in both districts was low. The morphology of An. vagus mosquitoes in this study was similar to the pictorial key. Sequencing and phylogenetic results with nDNA ITS2, mtDNA COI, and mtDNA ND6 markers showed that An. vagus Indonesia is a monophyletic species

Item Type:	Thesis (Doctoral)
Uncontrolled Keywords:	An. vagus, An. limosus, morphology, phylogenetic, monophyletic, and molecular.
Subjects:	Q Science > QL Zoology > QL461-599.82 Insects Q Science > QL Zoology > QL750-795 Animal behavior Q Science > QL Zoology > QL799-799.5 Morphology R Medicine > RC Internal medicine > RC109-216 Infectious and parasitic diseases
Divisions:	04-Faculty of Medicine > 11106-Biomedical Science (S2)
Depositing User:	Dalilah Dalilah
Date Deposited:	11 Sep 2024 01:35
Last Modified:	11 Sep 2024 01:35
URI:	http://repository.unsri.ac.id/id/eprint/154694

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