HUBUNGAN ANTARA KADAR VITAMIN D DENGAN KERUSAKAN ASAM DEOKSIRIBONUKLEAT PADA DOKTER YANG TERPAPAR SEVOFLURAN DI RUANG OPERASI: Analisis terhadap Fokus γ-H2AX, Frekuensi Mikronukleus Limfosit, dan Kadar 8-OH-DG

LESTARI, MAYANG INDAH and Murti, Krisna and Hafy, Zen (2025) HUBUNGAN ANTARA KADAR VITAMIN D DENGAN KERUSAKAN ASAM DEOKSIRIBONUKLEAT PADA DOKTER YANG TERPAPAR SEVOFLURAN DI RUANG OPERASI: Analisis terhadap Fokus γ-H2AX, Frekuensi Mikronukleus Limfosit, dan Kadar 8-OH-DG. Doctoral thesis, Sriwijaya University.

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Abstract

Introduction: Doctors who work in the operating room have a higher risk of deoxyribonucleic acid damage related to low vitamin D levels due to lack of sunlight exposure and high exposure to the inhalation anesthetic sevoflurane. This study aims to determine the association between vitamin D (25[OH]D) levels and deoxyribonucleic acid damage in terms of γ-H2AX foci, lymphocyte micronucleus frequency, and 8-OH-dg levels. Method: A cross-sectional study was conducted on 47 subjects in operating room of RS. Dr. Moh. Hoesin (RSMH) Palembang. Doctors aged 25-50 years, working in the operating room at least 20 hours/week, willing to take part in the study and sign the consent were included in the study; but those who were pregnant, with smoking habit (³11 cigarettes/day), alcohol drinker (³3 times/week), with a malignant disease, and chemotherapy or radiotherapy history were excluded. Subjects who withdrew were dropped out. Cutt off used in vitamin D deficient was 25 ng/ml. Data analysis includes descriptive and inferential analysis. Inferential analysis includes numerical comparative using independent t-test/Mann-Whitney and correlative Pearson/Spearman analysis. Result: Twelve subjects were with vitamin D deficiency and 35 subjects were not. The median of DNA damage parameters examined were higher in the vitamin D deficiency group but not statistically significant: γ-H2AX foci 2 (0-8) vs 1 (0-12); p = 0.803, lymphocyte micronucleus frequency 5.083 ± 0.633 vs 4.823 ± 0.463; p = 0.766, and 8-OHDG levels 45.93 (9.11-102.01) vs 36.68 (9.09-476.44); p = 0.643. There was a not significant negative correlation between vitamin D levels and γ-H2AX foci (r = -0.121; p = 0.416), lymphocyte micronucleus frequency (r = -0.171; p = 0.254), and 8-OH-DG levels (r = -0.191; p = 0.197). Conclusion: There was no association between vitamin D levels and γ-H2AX foci, lymphocyte micronucleus frequency, and 8-OH-dg levels.

Item Type: Thesis (Doctoral)
Uncontrolled Keywords: 8-OH-DG levels, γ-H2AX foci, lymphocyte micronucleus frequency, sevoflurane, vitamin D levels
Subjects: R Medicine > R Medicine (General) > R727-727.5 Medical personnel and the public. Physician and the public
Divisions: 04-Faculty of Medicine > 11106-Biomedical Science (S2)
Depositing User: Mayang Indah Lestari
Date Deposited: 22 Jan 2025 03:32
Last Modified: 22 Jan 2025 03:32
URI: http://repository.unsri.ac.id/id/eprint/163063

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