HUBUNGAN AREA MUTASI GEN rpoB Mycobacterium Tuberculosis DENGAN BACTERIAL LOAD SPUTUM DAN LUAS LESI RONTGEN DADA PASIEN TUBERKULOSIS PARU RESISTAN OBAT DI RSUP Dr MOHAMMAD HOESIN PALEMBANG

RACHMAN, MUHAMMAD ALIF FATHUR and Ahmad, Zen and Hafy, Zen (2024) HUBUNGAN AREA MUTASI GEN rpoB Mycobacterium Tuberculosis DENGAN BACTERIAL LOAD SPUTUM DAN LUAS LESI RONTGEN DADA PASIEN TUBERKULOSIS PARU RESISTAN OBAT DI RSUP Dr MOHAMMAD HOESIN PALEMBANG. Undergraduate thesis, Sriwijaya University.

Preview	Image RAMA_11802_04013782227002_cover.jpg - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (379kB) | Preview
	Text RAMA_11802_04013782227002.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy
	Text RAMA_11802_04013782227002_TURNITIN.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (12MB) | Request a copy
	Text RAMA_11802_04013782227002_0008036206_0029127201_01_front_ref.pdf - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (523kB)
	Text RAMA_11802_04013782227002_0008036206_0029127201_02.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy
	Text RAMA_11802_04013782227002_0008036206_0029127201_03.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (140kB) | Request a copy
	Text RAMA_11802_04013782227002_0008036206_0029127201_04.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (244kB) | Request a copy
	Text RAMA_11802_04013782227002_0008036206_0029127201_05.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (176kB) | Request a copy
	Text RAMA_11802_04013782227002_0008036206_0029127201_06.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (52kB) | Request a copy
	Text RAMA_11802_04013782227002_0008036206_0029127201_07_ref.pdf - Bibliography Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (163kB) | Request a copy
	Text RAMA_11802_04013782227002_0008036206_0029127201_08_lamp.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (563kB) | Request a copy

Abstract

Background: Mutations in the rpoB gene, which encodes the β-RNA-polymerase subunit, occur in an 81-bp area called the Rifampicin Resistant Determining Region (RRDR) in codons 507 to 533. This point mutation area is used to rapidly identify rifampicin-resistant isolates. Mutations in the rpoB gene area in MTb, in addition to having changes in phenotypic properties that cause resistance to the mechanism of action of the drug rifampicin. It turns out that there are also changes in the phenotypic properties of the MTb cell wall structure. The decrease in sulfoglycolipid structure, the increase in LAM structure, and the thicker mycolic acid layer make rpoB mutant MTb have an increased ability to avoid the human immune system. Thus, MTb is able to survive and proliferate, which can lead to higher virulence levels. This change in phenotypic properties has been carried out by several studies in vitro. However, no published studies have assessed the relationship clinically. Method: This study was a cross-sectional analytical observational study. Using a total sampling technique, all patients in the study population who met the inclusion and exclusion criteria during the period 2014-2023 at Dr Moh Hoesin Hospital Palembang were taken. Data were analysed bivariately and multivariately using SPSS. Result: 315 samples were collected through medical records and SITB data. There was a significant association between the mutation area of specific rpoB gene and sputum BTA index in the mutation area of probe D (p=0.003, PR 1.6) and probe E (p=0.001, PR 0.6). There was a significant association between specific rpoB gene mutation area and ct index in mutation area of probe D (p=0.001, PR 1.4) and probe E (p=0.001, PR 0.75). There was a significant association between specific rpoB gene mutation area and chest X-ray lesion area in the mutation area of probe B (p=0.039, PR 1.6). Conclusion: There is a relationship between certain MTb rpoB gene mutation areas with sputum bacterial load and chest X-ray lesion width area in drug-resistant pulmonary TB patients at RSMH Palembang. The rpoB gene mutation areas are probe areas D and E with high BTA index and high ct index. And, probe area B on chest X-ray lesion area.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Penyakit Dalam, Pulmonologi
Subjects:	R Medicine > RC Internal medicine > RC306-320.5 Tuberculosis
Divisions:	04-Faculty of Medicine > 11802-Internal Medicine (Sp
Depositing User:	Muhammad Alif Fathur Rachman
Date Deposited:	20 Jan 2025 03:26
Last Modified:	20 Jan 2025 03:26
URI:	http://repository.unsri.ac.id/id/eprint/164436

Actions (login required)

View Item