EFEK PEMBERIAN KETAMIN TERHADAP KONSENTRASI KALSIUM MITOKONDRIA PADA SEL NEURON DORSAL ROOT GANGLIA: ANALISIS KEMAMPUAN KETAMIN DALAM MENGHAMBAT DEPOLARISASI PADA SEL MODEL NYERI AKUT

ZAINAL, RIZAL and Irfannuddin, Irfannuddin and Legiran, Legiran and Ahmad, M Ramli (2024) EFEK PEMBERIAN KETAMIN TERHADAP KONSENTRASI KALSIUM MITOKONDRIA PADA SEL NEURON DORSAL ROOT GANGLIA: ANALISIS KEMAMPUAN KETAMIN DALAM MENGHAMBAT DEPOLARISASI PADA SEL MODEL NYERI AKUT. Doctoral thesis, Sriwijaya University.

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Abstract

Background: Ketamine, a multimodal drug used in pain management, functions as an N-methyl-D-aspartate (NMDA) receptor antagonist. It inhibits intracellular Ca2+ transport and has the potential to interfere with mitochondrial function. This study aimed to evaluate the effect of subanesthetic doses of ketamine on mitochondrial calcium concentration in a pain model cell. Methods: This in vitro experimental study was conducted at the Indonesian Medical Education and Research Institute (IMERI) from July 2023 to December 2024. A preliminary study was performed to establish an acute pain cell model by determining the optimal differentiation day of the F-11 cell line based on morphology and resting membrane potential, as well as the optimal dose of substance P (sP) to induce cell membrane depolarization, measured via patch clamp. Ketamine was then administered to DRG cells prior to pain induction with sP. Mitochondrial calcium concentration and mitochondrial membrane potential were assessed using a confocal microscope, while cell membrane potential activity was measured using patch clamp techniques. Toxicity testing was conducted beforehand to ensure cell viability at all ketamine doses. Results: The preliminary tests identified the fourth day as the most optimal for cell differentiation, while the effective dose range of sP for depolarizing DRG neurons was 0.3 µM to 30 µM. Pre-induction administration of ketamine inhibited depolarization caused by sP; however, no significant differences were observed in mitochondrial membrane potential or mitochondrial calcium concentration across the groups. Conclusion: Ketamine at subanesthetic doses does not impair mitochondrial activity but exhibits a depolarization-inhibiting effect on cell membranes. Keywords: Ketamine, substance P, F-11 cells, dorsal root ganglia, resting membrane potential, mitochondrial membrane potential, mitochondrial calcium

Item Type:	Thesis (Doctoral)
Uncontrolled Keywords:	Ketamin, substansi P, sel F-11, dorsal root ganglia, potensial membran istirahat (PMI), potensial membran mitokondria (PMM), kalsium mitokondria
Subjects:	R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions:	04-Faculty of Medicine > 11106-Biomedical Science (S2)
Depositing User:	Rizal Zainal
Date Deposited:	24 Jan 2025 01:50
Last Modified:	24 Jan 2025 01:50
URI:	http://repository.unsri.ac.id/id/eprint/166450

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