OPTIMASI PERTUMBUHAN BAKTERI TERMOFILIK Bacillus licheniformis AAL3 DAN Bacillus licheniformis SCL2 DALAM MEMPRODUKSI ENZIM KERATINASE

HASANAH, KHAIRATUL and Muharni, Muharni (2025) OPTIMASI PERTUMBUHAN BAKTERI TERMOFILIK Bacillus licheniformis AAL3 DAN Bacillus licheniformis SCL2 DALAM MEMPRODUKSI ENZIM KERATINASE. Undergraduate thesis, Sriwijaya University.

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Abstract

Keratin is a structural protein found in skin, hair, nails, and feathers in animals. The structure of keratin consisting of long and folded polypeptide chains, as well as the presence of disulfide bonds, gives keratin a very stable and difficult to decompose naturally, thus requiring an effective solution for its processing. Keratinolytic microorganisms have the ability to degrade keratin through the production of keratinase enzymes. This enzyme can break down keratin into smaller peptides and amino acids. One group of microorganisms that is promising for keratinase production is thermophilic bacteria. These bacteria can live and reproduce at high temperatures and have high enzyme stability at those temperatures. This study aims to optimize the growth of Bacillus licheniformis AAL3 and Bacillus licheniformis SCL2 bacteria isolated from the Air Putih Lebong hot spring, Bengkulu, in producing keratinase enzymes. The focus of this study is to determine the ability of bacteria to produce keratinase quantitatively and to identify optimal conditions, including temperature, pH, and nutrient sources that support enzyme production. This research was conducted from August to December 2024 at the Genetics and Biotechnology Laboratory, and the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indralaya. The research stages consisted of making liquid cultures, qualitative and quantitative tests of keratinolytic activity, then optimizing temperature, pH, and adding carbon and nitrogen sources. The results of the qualitative test of keratinolytic activity showed that Bacillus licheniformis AAL3 had a higher keratinolytic index (5.0) than Bacillus licheniformis SCL2 (1.5). Quantitative activity showed that Bacillus licheniformis AAL3 produced 1.0074 U/mL of keratinase enzyme, while Bacillus licheniformis SCL2 produced 0.7493 U/mL. The optimum temperature for keratinase production in both bacteria was found at 60°C, with a maximum pH at pH 9. The addition of a carbon source in the form of glucose and a nitrogen source in the form of peptone gave the best results in Bacillus licheniformis AAL3 bacteria, while Bacillus licheniformis SCL2 showed optimal results with the addition of sucrose and yeast extract. Keywords: Keratin, Keratinase Enzyme, Thermophilic Bacteria.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Keratin, Enzim Keratinase, Bakteri Termofilik.
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology > QR1-502 Microbiology Q Science > QR Microbiology > QR75-99.5 Bacteria
Divisions:	08-Faculty of Mathematics and Natural Science > 46201-Biology (S1)
Depositing User:	Khairatul Hasanah
Date Deposited:	19 Mar 2025 07:36
Last Modified:	19 Mar 2025 07:36
URI:	http://repository.unsri.ac.id/id/eprint/169400

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