HUBUNGAN KEKERABATAN FILOGENETIK TANAMAN KUNGKIL DAN MATOA (Pometia pinnata J.R.& G.Forst) BERDASARKAN SEKUEN ITS rDNA

USMAN, SALWA GHANIYU and Hanum, Laila (2025) HUBUNGAN KEKERABATAN FILOGENETIK TANAMAN KUNGKIL DAN MATOA (Pometia pinnata J.R.& G.Forst) BERDASARKAN SEKUEN ITS rDNA. Undergraduate thesis, Sriwijaya University.

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Abstract

Matoa (Pometia pinnata J.R.& G.Forst) is a plant from the Sapindaceae family with high genetic variation due to natural cross-pollination. This study aims to analyze the genetic variation and phylogenetic relationships between Kungkil and several Matoa variants (Red Matoa, Yellow Matoa, and Green Matoa) based on Internal Transcribed Spacer (ITS) rDNA sequences. The methods used include DNA isolation, quantitative and qualitative testing, DNA amplification using PCR, DNA sequencing, and phylogenetic analysis using BioEdit and MEGA 11 software. The results showed genetic variation in the ITS rDNA region with mutations at 35 points, consisting of 12 transversion mutations, 17 transition mutations, and 6 deletion mutations. Phylogenetic tree reconstruction revealed that Red Matoa, Yellow Matoa, and Green Matoa clustered together with Pometia pinnata voucher Chase 2135, while Kungkil was in the same cluster as Pometia pinnata voucher A4659. These findings indicate that Kungkil has genetic differences from other Matoa variants. This study provides valuable genetic information for the conservation and breeding of Matoa plants in South Sumatra. Further studies using additional molecular approaches are recommended to enrich the available genetic data.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Pometia pinnata, kungkil, ITS rDNA, hubungan kekerabatan filogenetik
Subjects:	Q Science > Q Science (General) > Q1-295 General Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany > QK495 Angiosperms
Divisions:	08-Faculty of Mathematics and Natural Science > 46201-Biology (S1)
Depositing User:	Salwa Ghaniyu Usman
Date Deposited:	24 Mar 2025 01:59
Last Modified:	24 Mar 2025 01:59
URI:	http://repository.unsri.ac.id/id/eprint/169953

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