PENGARUH RESTRIKSI KALORI DAN OLAHRAGA TERHADAP EKSPRESI CASPASE-3 PADA HATI TIKUS YANG MENGONSUMSI ALKOHOL

MAYA, RACHEL and Irfannuddin, Irfannuddin and Putri, Siti Sarahdeaz Fazzaura (2025) PENGARUH RESTRIKSI KALORI DAN OLAHRAGA TERHADAP EKSPRESI CASPASE-3 PADA HATI TIKUS YANG MENGONSUMSI ALKOHOL. Masters thesis, Sriwijaya University.

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Abstract

Global alcohol consumption is expected to rise by 17% over the next 13 years, which could increase the risk of liver disease due to oxidative stress, inflammation, and the activation of caspase-3, a key protein of apoptosis. While caloric restriction (CR) and exercise are known to reduce oxidative stress and caspase-3 levels, research on their effects on liver cell apoptosis during alcohol exposure is limited. This study utilized a post-test-only controlled group design with 30 male Wistar rats, of which 25 met the inclusion criteria for analysis. The groups included a control, an alcohol group, and groups receiving alcohol along with either CR, exercise, or both. Alcohol was administered at 2.5 mL of 20% ethanol, CR was set at 20% of the standard diet, and exercise involved using a hurdle running wheel at 10-12 m/min for 30 minutes each session. After six weeks, caspase-3 levels were assessed through immunohistochemical analysis and quantified using ImageJ, followed by statistical evaluation. Results showed a significant increase in caspase-3 expression in the alcohol group compared to the control (p < 0.001), with averages of 14.07% (control) versus 33.12% (alcohol). However, adding CR, exercise, or both did not significantly lower caspase-3 levels compared to the alcohol group alone (p > 0.05), with averages of 33.66%, 36.41%, and 42.12%, respectively. These results suggest that calorie restriction and exercise or combination of both do not effectively mitigate apoptosis induced by alcohol exposure. Alcohol appears to override any metabolic interventions, perpetuating apoptosis through oxidative stress and mitochondrial dysfunction.

Item Type: Thesis (Masters)
Uncontrolled Keywords: caspase-3, alkohol, restriksi kalori, olahraga, apoptosis hati
Subjects: Q Science > QP Physiology > QP1-(981) Physiology
R Medicine > R Medicine (General) > R5-920 Medicine (General)
R Medicine > RA Public aspects of medicine > RA773-788 Personal health and hygiene. Including clothing, bathing, exercise, travel, nutrition,
R Medicine > RB Pathology > RB151-214 Theories of disease. Etiology. Pathogenesis
R Medicine > RM Therapeutics. Pharmacology > RM214-258 Diet therapy. Dietary cookbooks
R Medicine > RM Therapeutics. Pharmacology > RM695-893 Physical medicine. Physical therapy. Including massage, exercise, occupational therapy, hydrotherapy, phototherapy, radiotherapy, thermotherapy, electrotherapy
Divisions: 04-Faculty of Medicine > 11106-Biomedical Science (S2)
Depositing User: Rachel Maya
Date Deposited: 09 May 2025 04:49
Last Modified: 09 May 2025 04:49
URI: http://repository.unsri.ac.id/id/eprint/171734

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