ISOLASI DAN UJI AKTIVITAS ENZIM LIPOKSIGENASE KACANG KEDELAI

CHRISTIANNI M S, CHRISTIANNI M S and Julinar, Julinar and Syarif, Nirwan (2015) ISOLASI DAN UJI AKTIVITAS ENZIM LIPOKSIGENASE KACANG KEDELAI. Undergraduate thesis, Sriwijaya University.

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Abstract

Test has been carried out isolation and enzyme activity of soybean lipoxygenase. Fat-free soy bean flour is made by soaking using chloroform methanol solvent to produce a fat-free soy flour amounted to 87,17%. Lipoxygenase enzyme was isolated by extraction using distilled water so that the lipoxygenase enzyme extracts obtained by 16 mL/g. Enzyme activity assay using methods Hamberg & Samuelsson in order to obtain lipoxygenase enzyme activity of 1460 Units/mL. The protein content is measured by the enzyme extract biuret method and obtained the protein content of 2,72 mg/mL. Subsequently extract characterized enzymes and enzymes get optimum conditions at the time of 5 minutes, the temperature 25 degrees celcius, the concentration of the substrate 2 mL and pH 9 with activity of 3428 units/mL.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Lipoxygenase enzyme, fat-free soy flour, enzyme activity levels of protein, the enzyme optimum conditions
Subjects:	Q Science > QD Chemistry > QD1-999 Chemistry Q Science > QD Chemistry > QD241-441 Organic chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Mrs Dies Meirita Sari
Date Deposited:	20 Nov 2019 04:44
Last Modified:	20 Nov 2019 04:44
URI:	http://repository.unsri.ac.id/id/eprint/17290

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