AKTIVITAS ANTIBAKTERI NANOPARTIKEL EKSTRAK FUNGI ENDOFIT BUAH KABAU (Archidendron bubalinum (Jack) I.C. Nielsen) TERHADAP Escherichia coli ATCC 25922 DAN Staphylococcus aureus ATCC 25923

MAYORI, SEPTINA ELSA and Widjajanti, Hary (2025) AKTIVITAS ANTIBAKTERI NANOPARTIKEL EKSTRAK FUNGI ENDOFIT BUAH KABAU (Archidendron bubalinum (Jack) I.C. Nielsen) TERHADAP Escherichia coli ATCC 25922 DAN Staphylococcus aureus ATCC 25923. Undergraduate thesis, Sriwijaya University.

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Abstract

Endophytic fungi are microorganisms that live within plant tissues without causing damage to their host and are known as producers of bioactive compounds with pharmacological potential, including antibacterial activity. The kabau fruit (Archidendron bubalinum (Jack) I. C. Nielsen) is one of the sources of endophytic fungi that produce bioactive compounds proven to have antibacterial potential. However, the use of conventional bioactive compounds often has limitations, such as bacterial resistance and low stability. To address this issue, this study utilizes nanoparticle technology to enhance the antibacterial effectiveness of kabau fruit endophytic fungi extract against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. This study aims to develop and characterize nanoparticles of kabau fruit endophytic fungi extract, including particle size, polydispersity index (PDI), and zeta potential, as well as to evaluate their antibacterial activity against the test bacteria. The research was conducted from October to December 2024 at the Microbiology Laboratory, Genetics and Biotechnology Laboratory, and Pharmaceutical Technology Laboratory, Faculty of Mathematics and Natural Sciences, Sriwijaya University. The research stages included rejuvenation of kabau fruit endophytic fungi, cultivation and extraction of secondary metabolites, thin-layer chromatography (TLC) analysis, bioautography assay, preparation and characterization of nanoparticles from kabau fruit endophytic fungi extract, and antibacterial activity testing. The results showed that nanoparticles of endophytic fungi extracts from Fusarium sp., Daldinia sp., and Diaporthe sp. had particle sizes of 91 nm, 26.44 nm, and 17.26 nm, respectively, with polydispersity indices (PDI) of 0.579, 0.694, and 0.356, and zeta potentials of -7.15 mV, -9.44 mV, and -3.20 mV, respectively. Antibacterial activity tests demonstrated that nanoparticles of endophytic fungi extracts were able to inhibit bacterial growth, with a maximum inhibition zone diameter of 24 mm. In conclusion, nanoparticles of kabau fruit endophytic fungi extract exhibit stable physicochemical characteristics and show significant antibacterial activity against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Endophytic fungi, Archidendron bubalinum (Jack) I. C. Nielsen, Nanoparticles, Antibacterial
Subjects:	Q Science > QR Microbiology
Divisions:	08-Faculty of Mathematics and Natural Science > 46201-Biology (S1)
Depositing User:	Septina Elsa Mayori
Date Deposited:	22 May 2025 04:55
Last Modified:	22 May 2025 04:55
URI:	http://repository.unsri.ac.id/id/eprint/173597

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