PERBANDINGAN METODE KATO-KATZ DENGAN PCR UNTUK MENDIAGNOSIS INFEKSI SOIL TRANSMITTED HELMINTHS

RISTI, SANELY and Liberty, Iche Andriyani and Dalilah, Dalilah (2025) PERBANDINGAN METODE KATO-KATZ DENGAN PCR UNTUK MENDIAGNOSIS INFEKSI SOIL TRANSMITTED HELMINTHS. Masters thesis, Sriwijya University.

Preview	Image RAMA_11106_04112622327008_cover.jpg - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (149kB) | Preview
	Text RAMA_11106_04112622327008.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (3MB) | Request a copy
	Text RAMA_11106_04112622327008_TURNITIN.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (10MB) | Request a copy
	Text RAMA_11106_04112622327008_0007029001_0021118401_01_front_ref.pdf - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (832kB)
	Text RAMA_11106_04112622327008_0007029001_0021118401_02.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy
	Text RAMA_11106_04112622327008_0007029001_0021118401_03.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (683kB) | Request a copy
	Text RAMA_11106_04112622327008_0007029001_0021118401_04.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (490kB) | Request a copy
	Text RAMA_11106_04112622327008_0007029001_0021118401_05.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (187kB) | Request a copy
	Text RAMA_11106_04112622327008_0007029001_0021118401_06_ref.pdf - Bibliography Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (432kB) | Request a copy
	Text RAMA_11106_04112622327008_0007029001_0021118401_07_lamp.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy

Abstract

Soil-Transmitted Helminth (STH) infections are a public health problem in many developing countries, particularly in subtropical and tropical regions, including Indonesia. Intestinal parasite are among the most common infections affecting individuals with HIV due to compromised immune function. Accurate diagnostic methods are essential for proper detection of these infections. This study aimed to compare the accuracy of the Kato-Katz method and PCR in diagnosing STH infections among HIV patients in Palembang, Indonesia. This observational cross-sectional study involved 34 HIV-positive subjects. Stool samples from each participant were examined using both Kato-Katz and PCR methods. Data were analyzed using diagnostic test parameters, and Kappa test. The prevalence of STH infection detected by the Kato-Katz method was 94.12%, while the PCR method detected 70.59%. Kato-Katz identified only Ascaris lumbricoides, whereas PCR detected Ascaris lumbricoides, Necator americanus, and including mixed infections. The sensitivity of PCR was 71.90%, specificity 50.00%, positive predictive value (PPV) 95.88%, negative predictive value (NPV) 10.00%, and diagnostic accuracy 70.58%. The agreement between methods was very weak, as shown by a low Kappa value 0,761. PCR was sensitive in detecting STH infections, particularly in cases of light or mixed infections, but it has limitations in identifying negative cases. A combination of Kato-Katz and PCR is recommended to achieve more accurate diagnostic results, especially in immunocompromised populations such as people living with HIV. Keywords: Soil-Transmitted Helminths, HIV, Kato-Katz, PCR, parasitic diagnosis

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Soil-Transmitted Helminths, HIV, Kato-Katz, PCR, diagnosis parasit
Subjects:	R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions:	04-Faculty of Medicine > 11106-Biomedical Science (S2)
Depositing User:	Sanely Risti
Date Deposited:	23 May 2025 02:00
Last Modified:	23 May 2025 02:00
URI:	http://repository.unsri.ac.id/id/eprint/173689

Actions (login required)

View Item