DISERTASI PERBANDINGAN PROPORSI KEJADIAN APOPTOSIS, NEKROPTOSIS DAN PIROPTOSIS DALAM PROSES KEMATIAN SEL T CD4 DENGAN PENANDA CASPASE 3, RIPK3 DAN CASPASE 1 PADA PASIEN HIV/AIDS NAIF DI RS MOHAMMAD HOESIN PALEMBANG

HUDARI, HARUN and Saleh, Irsan and Anwar, Chairil and Ali, Zulkhair (2025) DISERTASI PERBANDINGAN PROPORSI KEJADIAN APOPTOSIS, NEKROPTOSIS DAN PIROPTOSIS DALAM PROSES KEMATIAN SEL T CD4 DENGAN PENANDA CASPASE 3, RIPK3 DAN CASPASE 1 PADA PASIEN HIV/AIDS NAIF DI RS MOHAMMAD HOESIN PALEMBANG. Doctoral thesis, Sriwijaya University.

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Abstract

ABSTRAK PERBANDINGAN PROPORSI KEJADIAN APOPTOSIS, NEKROPTOSIS DAN PIROPTOSIS DALAM PROSES KEMATIAN SEL T CD4 DENGAN PENANDA CASPASE 3, RIPK3 DAN CASPASE 1 PADA PASIEN HIV/AIDS NAIF DI RS MOHAMMAD HOESIN PALEMBANG Latar Belakang: Penurunan jumlah sel T CD4 merupakan ciri khas infeksi HIV yang berkontribusi pada gangguan sistem imun dan perkembangan AIDS. Mekanisme kematian sel T CD4 melibatkan berbagai jalur seperti apoptosis, nekroptosis, dan piroptosis, yang masing-masing memiliki penanda molekuler khusus, yaitu caspase 3, RIPK3, dan caspase 1. Penelitian ini bertujuan untuk membandingkan proporsi kejadian ketiga mekanisme kematian tersebut pada pasien HIV/AIDS naif. Metode: Penelitian observasional cross-sectional ini melibatkan pasien HIV/AIDS naif di RS Mohammad Hoesin Palembang. Proses kematian sel T CD4 dianalisis menggunakan pemeriksaan flow cytometry terhadap caspase 3 (apoptosis), RIPK3 (nekroptosis), dan caspase 1 (piroptosis). Hasil: Proporsi kematian sel T CD4 melalui piroptosis (positif caspase 1) mencapai 94 %, jauh lebih tinggi dibandingkan apoptosis (6,620 %) dan nekroptosis (0,265 %). Perbedaan ini signifikan dan menunjukkan dominasi mekanisme inflamasi dalam kematian sel T CD4. Kesimpulan: Piroptosis merupakan mekanisme utama kematian sel T CD4 pada pasien HIV/AIDS naif di RS Mohammad Hoesin Palembang. Pemahaman mekanisme ini penting untuk pengembangan strategi terapi imunomodulator yang lebih efektif. Kata kunci: Apoptosis, Nekroptosis, Piroptosis, Sel T CD4, Caspase 3, RIPK3, Caspase 1, HIV/AIDS Naif. ABSTRACT Comparison of the Proportion of Apoptosis, Necroptosis, and Pyroptosis Occurrence in CD4 T Cell Death Process Using Caspase 3, RIPK3, and Caspase 1 Markers in Naive HIV/AIDS Patients at Mohammad Hoesin Hospital Palembang Background. Depletion of CD4 T cells is a hallmark of HIV infection, contributing to immune dysfunction and progression to AIDS. CD4 T cell death occurs through multiple pathways including apoptosis, necroptosis, and pyroptosis, marked respectively by caspase 3, RIPK3, and caspase 1. This study aimed to compare the proportions of these mechanisms in naïve HIV/AIDS patients. Methods. This cross-sectional observational study involved naïve HIV/AIDS patients at Mohammad Hoesin Hospital Palembang. CD4 T cell death pathways were analyzed using flow cytometry for caspase 3 (apoptosis), RIPK3 (necroptosis), and caspase 1 (pyroptosis). Results. Pyroptosis (caspase 1 positive) accounted for 94 % of CD4 T cell death, which was significantly higher than apoptosis (6,620 %) and necroptosis (0,265 %). These findings emphasize the inflammatory nature of CD4 T cell loss in HIV pathogenesis. Conclusion. Pyroptosis is the dominant mechanism of CD4 T cell death in naïve HIV/AIDS patients at Mohammad Hoesin Hospital Palembang. Understanding these mechanisms is essential for developing more effective immunomodulatory therapies. Keywords. Apoptosis, Necroptosis, Pyroptosis, CD4 T cells, Caspase 3, RIPK3, Caspase 1, Naïve HIV/AIDS.

Item Type:	Thesis (Doctoral)
Uncontrolled Keywords:	Apoptosis, Nekroptosis, Piroptosis, Sel T CD4, Caspase 3, RIPK3, Caspase 1, HIV/AIDS Naif. Apoptosis, Necroptosis, Pyroptosis, CD4 T cells, Caspase 3, RIPK3, Caspase 1, Naïve HIV/AIDS.
Subjects:	R Medicine > R Medicine (General) > R5-920 Medicine (General) R Medicine > RC Internal medicine > RC31-1245 Internal medicine R Medicine > RC Internal medicine > RC581-951 Specialties of internal medicine R Medicine > RC Internal medicine > RC81-82 Popular medicine
Divisions:	04-Faculty of Medicine > 11106-Biomedical Science (S2)
Depositing User:	Harun Hudari
Date Deposited:	17 Jul 2025 04:31
Last Modified:	17 Jul 2025 04:31
URI:	http://repository.unsri.ac.id/id/eprint/178786

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