INDUKSI KULTUR KALUS RUMPUT LAUT HALYMENIA DURVILLEI MELALUI SUPLEMENTASI ZAT PENGATUR TUMBUH YANG BERBEDA

SAPUTRI, ANGGI and Muhammad, Hendri and Purwiyanto, Anna Ida Sunaryo (2025) INDUKSI KULTUR KALUS RUMPUT LAUT HALYMENIA DURVILLEI MELALUI SUPLEMENTASI ZAT PENGATUR TUMBUH YANG BERBEDA. Undergraduate thesis, Sriwijaya University.

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Abstract

Halymenia durvillei is a red seaweed species with considerable potential for commercial cultivation in Indonesia. Tissue culture has emerged as a promising method to enhance its biomass production. This study aimed to analyze the most effective combination of plant growth regulators (PGRs) for callus induction and to examine the morphological characteristics of the induced callus. The research was conducted from January to April 2025 using an experimental design supported by descriptive and statistical analyses. Five combinations of PGRs, comprising 2,4-dichlorophenoxyacetic acid (2,4-D), thidiazuron (TDZ), and gibberellic (GA₃), were tested in treatments A through E. All treatments successfully induced callus formation, with effectiveness rates ranging from 73.33% to 100%. Treatment D (2,4-D 0.5 mg/L + TDZ 2 mg/L + GA₃ 0.6 mg/L) resulted in 100% viable callus and achieved the highest average rank based on biological observations, although statistical analysis indicated no significant differences among treatments (Asymp. Sig. = 0.245). The induced callus was white (score 1), compact (non-friable), and microscopic examination suggested it consisted of moniliform filamentous cells.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Halymenia durvillei, Kultur Jaringan, Kalus, ZPT
Subjects: Q Science > QH Natural history > QH1-278.5 Natural history (General)
T Technology > TP Chemical technology > TP248.13-248.65 Biotechnology
Divisions: 08-Faculty of Mathematics and Natural Science > 54241-Marine Science (S1)
Depositing User: Anggi Saputri
Date Deposited: 28 Jul 2025 05:05
Last Modified: 28 Jul 2025 05:05
URI: http://repository.unsri.ac.id/id/eprint/181133

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