HIDROLISIS ENZIMATIK SELULOSA DARI LIMBAH SERBUK KAYU KARET YANG DIBERI PRAPERLAKUAN ALKALIN MICROWAVE ASSISTED

RIDWAN, MUHAMAD and Novia, Novia and Selpiana, Selpiana (2025) HIDROLISIS ENZIMATIK SELULOSA DARI LIMBAH SERBUK KAYU KARET YANG DIBERI PRAPERLAKUAN ALKALIN MICROWAVE ASSISTED. Undergraduate thesis, Sriwijaya University.

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Abstract

Serbuk gergaji kayu karet mengandung selulosa, yang berfungsi sebagai bahan baku untuk produksi bioetanol. Selulosa dari kayu karet yang telah diolah terlebih dahulu ditransformasikan menjadi gula pereduksi melalui hidrolisis enzimatik menggunakan enzim yang berasal dari Aspergillus niger. Penelitian ini bertujuan untuk mengklarifikasi kinetika Hidrolisis enzimatik selama durasi hidrolisis 5, 10, 15, 20, dan 25 jam pada rasio enzim terhadap substrat 1:20, 2:20, 3:20, 4:20, 5:20, dan 6:20 (v/b). Hasil penelitian menunjukkan bahwa, pada rasio enzim terhadap substrat 6:20 (v/b) dan waktu hidrolisis 25 jam, jumlah maksimum gula pereduksi adalah sekitar 3,15 g L−1. Aktivitas FPase adalah 255,1672 U mL-1, sedangkan aktivitas CMCase mencapai 235,9105. Model kinetik Michaelis–Menten menyatakan bahwa laju maksimum berkisar antara 6,50×10−7 hingga 2,6×10−6 Mol L−1 s−1, dan laju reaksi menghasilkan konstanta Michaelis antara 0,0051 dan 0,0055.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Kinetika hidrolisis enzimatik, Model Michaelis-Menten, Pretreatment Alkali Microwave-Assisted, Serbuk kayu karet, Gula reduksi.
Subjects:	Q Science > QD Chemistry > QD1-999 Chemistry Q Science > QD Chemistry > QD241-441 Organic chemistry Q Science > QD Chemistry > QD415-436 Biochemistry Q Science > QD Chemistry > QD625-655 Radiation chemistry T Technology > TD Environmental technology. Sanitary engineering > TD1-1066 Environmental technology. Sanitary engineering T Technology > TD Environmental technology. Sanitary engineering > TD169-171.8 Environmental protection T Technology > TD Environmental technology. Sanitary engineering > TD172-193.5 Environmental pollution T Technology > TD Environmental technology. Sanitary engineering > TD194-195 Environmental effects of industries and plants T Technology > TD Environmental technology. Sanitary engineering > TD896-899 Industrial and factory wastes T Technology > TP Chemical technology > TP248.13-248.65 Biotechnology
Divisions:	03-Faculty of Engineering > 24101-Chemical Engineering (S2)
Depositing User:	Muhamad Ridwan
Date Deposited:	29 Jul 2025 07:41
Last Modified:	29 Jul 2025 07:41
URI:	http://repository.unsri.ac.id/id/eprint/181443

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