Amebocyte lysate Asian horseshoe crab for bacterial endotoxin test from the estuary waters of Banyuasin, South Sumatra

Mustopa, Apon Zainal and Fatimah, Fatimah and Fauziyah, Fauziyah and Sari, Nita Puspita and Agustriani, Fitri and Priyanto, Langgeng and Rozirwan, Rozirwan and Melki, Melki (2023) Amebocyte lysate Asian horseshoe crab for bacterial endotoxin test from the estuary waters of Banyuasin, South Sumatra. In: Sriwijaya International Conference on Basic and Applied Sciences 2021.

Text amebocyte mimi 2023.pdf - Published Version Available under License Creative Commons Public Domain Dedication. Download (1MB)

Abstract

Horseshoe crab blood contains amebocyte lysate which is very important in the process of detecting bacterial endotoxins. When endotoxin enters the bloodstream, the inflammatory response of our immune system causes damage to blood vessels. The purpose of this study was to determine the ability of Asian Horseshoe Crab (AHC) amebocyte lysate from the Estuary waters of Banyuasin, South Sumatra to detect endotoxin bacteria. A sampling of horseshoe crabs was carried out in March 2021. The 28 individual horseshoe crabs were identified using morphometrics and 10 ml of blood was taken from each individual. Afterward, the horseshoe crabs were released back into their habitat. The bacterial endotoxin test (BET) is based on the qualitative method (Gel Clot) and quantitative method (Chromogenic). The bacterial endotoxin concentrations used were 1 EU/ml, 0.5 EU/ml, 0.25 EU/ml and 0.125 EU/ml. The identification species showed 2 species of AHC, namely Carcinoscorpius rotundicauda and Tachypleus gigas. Out of 28 individuals for amebocyte lysate assay resulted 4 individuals were able to detect the presence of endotoxin bacteria using the Gel Clot method. It is characterized by the presence of a weak coagulant with a maximum endurance of 12 seconds of incubation. The Chromogenic method can only detect one individual of T. gigas with a very strong correlation (r = 0.905) within 60 minutes of incubation. The results showed that the amebocyte lysate of AHC from the Estuary waters of Banyuasin, South Sumatra could detect the presence of bacterial endotoxin.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	#3 Repository of Lecturer Academic Credit Systems (TPAK) > Conference or Workshop
Divisions:	08-Faculty of Mathematics and Natural Science > 54241-Marine Science (S1)
Depositing User:	Fitri Agustriani
Date Deposited:	07 Aug 2025 03:14
Last Modified:	07 Aug 2025 03:14
URI:	http://repository.unsri.ac.id/id/eprint/182193

Actions (login required)

View Item