PENAMBAHAN MADU PADA BAHAN EKSTENDER DALAM KRIOPRESERVASI SPERMA IKAN TAMBAKAN (HELOSTOMA TEMMINCKII)

ABROR, MUHAMMAD and Yonarta, Danang (2025) PENAMBAHAN MADU PADA BAHAN EKSTENDER DALAM KRIOPRESERVASI SPERMA IKAN TAMBAKAN (HELOSTOMA TEMMINCKII). Undergraduate thesis, Sriwijaya University.

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Abstract

The process of preserving and storing sperm is called cryopreservation process. The success of the sperm cryopreservation process is determined by several factors including extender material, fisiological solution and freezing rates. One of the extender ingredients that can be used is honey. Honey contains antioxidants and has the ability to increase extracellular osmotic activity which helps protect cells from freezing and thawing damage. This study aims to determine the best dose of honey addition as an extender in the cryopreservation of kissing gourami sperm. The cryopreservation process was carried out at the Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak Sembawa. The preparation of cryomedia solution and maintenance of kissing gourami broodstock were carried out at the Aquaculture Laboratory and Experimental Ponds of the Aquaculture Study Program, Faculty of Agriculture, Universitas Sriwijaya which was carried out in July-September 2024. This study was designed used a completely randomized design consisting of (P0) 100% lactate ringer solution without honey, (P1) 0.25% honey + 99.75% lactate ringer solution, (P2) 0.5% honey + 99.50% lactate ringer solution, (P3) 0.75% honey + 99.25% lactate ringer solution. The results showed that fresh kissing gourami sperm had a sperm volume value of 0.6 mL, pH 7, milky white color, sperm concentration of 1.11x109 cell mL-1, sperm mass movement with a score of 5 and sperm viability of 93.2%. In the cryopreservation process for 14 days, (P2) with a honey dose of 0.5% was obtained as the best treatment which resulted in sperm mass movement with a score of 4, duration of sperm movement 112 seconds and sperm viability of 76.18%.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	ekstender, ikan tambakan, kriopreservasi sperma, madu
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture
Divisions:	05-Faculty of Agriculture > 54243-Aquaculture (S1)
Depositing User:	Muhammad Abror
Date Deposited:	26 Aug 2025 07:49
Last Modified:	26 Aug 2025 07:49
URI:	http://repository.unsri.ac.id/id/eprint/183287

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