MEKANISME DISBIOSIS MIKROBIOTA USUS PADA SINDROM OVARIUM POLIKISTIK MELALUI PEMERIKSAAN JUMLAH MIKROBA, ZONULIN, SHORT CHAIN FATTY ACIDS, INSULIN-LIKE GROWTH FACTOR-1,HOMEOSTASIS MODEL ASSESSMENT OF INSULIN RESISTANCE DAN JUMLAH FOLIKEL OVARIUM

USMAN, FATIMAH and Saleh, H. Muhammad Irsan and Effendi, Kms. Yusuf and Lestari, Peby Maulina (2025) MEKANISME DISBIOSIS MIKROBIOTA USUS PADA SINDROM OVARIUM POLIKISTIK MELALUI PEMERIKSAAN JUMLAH MIKROBA, ZONULIN, SHORT CHAIN FATTY ACIDS, INSULIN-LIKE GROWTH FACTOR-1,HOMEOSTASIS MODEL ASSESSMENT OF INSULIN RESISTANCE DAN JUMLAH FOLIKEL OVARIUM. Doctoral thesis, Sriwijaya University.

	Image RAMA_11006_04013622328005_cover.jpeg - Cover Image Available under License Creative Commons Public Domain Dedication. Download (439kB)
	Text RAMA_11006_04013622328005.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (9MB) | Request a copy
	Text RAMA_11006_04013622328005_TURNITIN.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (41MB) | Request a copy
	Text RAMA_11006_04013622328005_0029096608_8828230017_8845330017_0013067302_000510621101_0015046204_01_front_ref.pdf - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (1MB)
	Text RAMA_11006_04013622328005_0029096608_8828230017_8845330017_0013067302_000510621101_0015046204_02.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (2MB) | Request a copy
	Text RAMA_11006_04013622328005_0029096608_8828230017_8845330017_0013067302_000510621101_0015046204_03.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (652kB) | Request a copy
	Text RAMA_11006_04013622328005_0029096608_8828230017_8845330017_0013067302_000510621101_0015046204_04.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (536kB) | Request a copy
	Text RAMA_11006_04013622328005_0029096608_8828230017_8845330017_0013067302_000510621101_0015046204_05.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (429kB) | Request a copy
	Text RAMA_11006_04013622328005_0029096608_8828230017_8845330017_0013067302_000510621101_0015046204_06.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (279kB) | Request a copy
	Text RAMA_11006_04013622328005_0029096608_8828230017_8845330017_0013067302_000510621101_0015046204_07_ref.pdf - Bibliography Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (293kB) | Request a copy
	Text RAMA_11006_04013622328005_0029096608_8828230017_8845330017_0013067302_000510621101_0015046204_08_lamp.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (4MB) | Request a copy

Abstract

Mechanism of Gut Microbiota Dysbiosis in Polycystic Ovary Syndrome Through the Assessment of Microbial Count, Zonulin, Short Chain Fatty Acids, Insulin-like Growth Factor-1, Homeostasis Model Assessment of Insulin Resistance, and Ovarian Follicle Count Background: Polycystic ovary syndrome (PCOS) with insulin resistance is a complex endocrine disorder involving multifactorial interactions between metabolic, hormonal, and gut microbial systems. Gut microbiota dysbiosis may play a role in the pathophysiology of PCOS through the gut-brain-gonad axis. This study aimed to analyze the relationship between gut microbiota dysbiosis, intestinal permeability (Zonulin), microbial metabolites (Short Chain Fatty Acids/SCFAs), IGF-1 regulation, insulin resistance (HOMA-IR), and folliculogenesis in a rat model of PCOS with insulin resistance. Methods: This experimental study involved female Wistar rats divided into a control group and a PCOS-insulin resistance model group. Gut microbiota analysis was conducted along with measurements of Zonulin, SCFAs, IGF-1, HOMA-IR levels, and ovarian follicle count. Structural pathway analysis was used to evaluate the contribution of each parameter within the gut-brain-gonad axis framework. Results: A significant decrease in Lactobacillus sp. count and SCFA levels was observed, along with increased levels of Zonulin, IGF-1, and HOMA-IR in the PCOS model group. The number of immature follicles was significantly elevated. Pathway analysis revealed a significant sequential relationship: PCOS → Lactobacillus sp. → SCFAs → IGF-1 → follicle count, supporting the involvement of gut microbiota dysbiosis in the pathophysiology of PCOS. Conclusion: Gut microbiota dysbiosis plays a crucial role in metabolic disturbances and impaired folliculogenesis in a PCOS model with insulin resistance. The gut-brain-gonad axis provides a novel conceptual framework for understanding PCOS pathophysiology and offers a foundation for developing microbiota-targeted non-hormonal therapeutic strategies. Keywords: Polycystic ovary syndrome, gut microbiota dysbiosis, SCFAs, Zonulin, IGF-1, HOMA-IR, gut-brain-gonad axis

Item Type:	Thesis (Doctoral)
Uncontrolled Keywords:	Sindrom ovarium polikistik, disbiosis mikrobiota usus, SCFAs, Zonulin, IGF-1, HOMA-IR, gut-brain-gonad axis
Subjects:	R Medicine > R Medicine (General) > R856-857 Biomedical engineering. Electronics. Instrumentation > R857.M3.B56854 Biomedical materials. Stem cells--Therapeutic use. Regenerative medicine--Materials. TECHNOLOGY & ENGINEERING / Material Science. MEDICAL / Biotechnology
Divisions:	04-Faculty of Medicine > 11006-Biomedical Science (S3)
Depositing User:	Fatimah Usman
Date Deposited:	17 Sep 2025 05:30
Last Modified:	17 Sep 2025 05:30
URI:	http://repository.unsri.ac.id/id/eprint/184054

Actions (login required)

View Item