Isolasi Metabolit Sekunder dari Jamur Endofitik Akar Tumbuhan Sambiloto (Andrographis paniculata Nees)

Elfita, Elfita and Munawar, Munawar (2014) Isolasi Metabolit Sekunder dari Jamur Endofitik Akar Tumbuhan Sambiloto (Andrographis paniculata Nees). In: Prosiding Seminar Nasional dan Rapat Tahunan Bidang MIPA 2014. FMIPA IPB Bagor, Bogor, pp. 2-7. ISBN 78-602-70491-0-9

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Abstract

Endophytic fungi are important sources of bioactive natural products with enormous potential for the discovery of new molecules for drug discovery, industrial use and agricultural applications. This study was conducted on the isolation of secondary metabolite from the endophytic fungi of selected plants sambiloto. Three endophytic fungi from root of sambiloto were isolated and purified. One of the Unidentified fungi (Sb-1) was cultured in 5L Potato Dextrose Broth (PDB) medium at room temperature (no shaking) for 8 weeks. The culture broth was filtered to remove mycelia. The culture media was then extracted in 5 L ethyl acetate (2 times), followed by filtration and then taken to evaporation. The extract was separated into the fractions by column chromatography (CC) on silica gel. The fractions was further separated by silica gel column chromatography to give one aromatic compound. The molecular structure was identified on the basis of spectroscopic UV analysis

Item Type: Book Section
Subjects: Q Science > QD Chemistry > QD1-999 Chemistry
Divisions: Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User: Dr. Elfita, M.Si
Date Deposited: 16 Jan 2020 07:02
Last Modified: 16 Jan 2020 07:02
URI: http://repository.unsri.ac.id/id/eprint/24259

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