UJI EFIKASI BIO-INSEKTISIDA Bacillus thuringiensis DENGAN PERLAKUAN PEMISAHAN PROTEIN, SUPERNATAN (SPORA) DAN LARUTAN CAMPURAN TERHADAP RAYAP Macrotermes gilvus (ISOPTERA: TERMITIDAE)

AHMAD, RISKAL and Yulia, Pujiastuti (2019) UJI EFIKASI BIO-INSEKTISIDA Bacillus thuringiensis DENGAN PERLAKUAN PEMISAHAN PROTEIN, SUPERNATAN (SPORA) DAN LARUTAN CAMPURAN TERHADAP RAYAP Macrotermes gilvus (ISOPTERA: TERMITIDAE). Undergraduate thesis, Sriwijaya University.

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Abstract

Soil termites (Macrotermes gilvus) are one of the destructive insect pests that cause huge losses. These termites are the main pests on perennial plantation crops, therefore control must be carried out. One of the effective controls and also does not pollute nature is Bacillus thuringiensis bioinsecticide. In the stage of making bioinsecticide based on B. thuringiensis, the separation of supernatant and protein is carried out, the supernatant contains spores from bacteria and protein contains protein crystals from bacteria. The aim of this study was to determine the efficacy of protein, supernatant (spore), and mixed solution of B. thuringiensis bioinsecticide in controlling M. gilvus termites. This research was arranged in an experiment that was designed with a factorial randomized design (FRD), with 2 factors. The first factor’s ten isolates and second factor’s bioinsecticide treatment factors. Bioinsecticide treatment was separation of protein and supernatant. The isolates used were isolate collections from the Entomology Laboratory of Plant Pests and Diseases, and were redistributed on NGKG media. In making bioinsecticide B. thuringiensis prepared media is bio urine, molasses, and nutrient broth media (NB). Before the application is carried out the separation of protein and supernatant by centrifugation. Bioinsecticide was applied to termite feed with a concentration of 0,2 ml of bioinsecticide solution and 20 ml of aquades. The highest spore density was highest in the manufacture of 72 hour pesticides that was on the SMR4 isolate with an average of 11,23x1012 spores / ml. The mean spore density in the supernatant treatment was MSP isolate with an average of 6,00x1012 spores / ml and the highest spore density of the mixed solution was 11,24x1012 spores / ml isolates. The highest average mortality occurred in isolates KJ3P1 and the highest average mortality in the treatment occurred in the application of supernatant. The highest LT50 for protein application is SMR4 isolate with the time needed to kill 50% of test insects is 1s,025 days, for the highest LT50 supernatant application is KJ3P1 isolate with the time needed to kill 50% of test insects that is 0,947 days and the highest application of mixed solution, LT50 is highest that is KJ3P1 isolate with the time needed to kill 50% of the test insects which is 0,921 days. For weight reduction, the percentage of depreciation was calculated, the highest depreciation (other than control) occurred in the treatment of SASU isolates applying protein with a shrinkage of 4,2%.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	M.gilvus,b. thuringiensis, mortalitas, bioinsektisida,LT 50, supernatan, protein,larutan campuran
Subjects:	S Agriculture > S Agriculture (General) > S1-(972) Agriculture (General) S Agriculture > SB Plant culture > SB950-990.5 Pest control and treatment of diseases. Plant protection
Divisions:	05-Faculty of Agriculture > 54295-Plant Protection (S1)
Depositing User:	Users 4504 not found.
Date Deposited:	27 Jan 2020 04:53
Last Modified:	27 Jan 2020 04:53
URI:	http://repository.unsri.ac.id/id/eprint/25759

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