UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN KOPI ROBUSTA (Coffea canephora) TERHADAP TIKUS JANTAN GALUR WISTAR DENGAN METODE FIXED DOSE PROCEDURE

ANDHINI, MONA RISKA and Herlina, Herlina and Amriani, Annisa (2020) UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN KOPI ROBUSTA (Coffea canephora) TERHADAP TIKUS JANTAN GALUR WISTAR DENGAN METODE FIXED DOSE PROCEDURE. Undergraduate thesis, Sriwijaya University.

	Text RAMA_48201_08061381520037.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (2MB) | Request a copy
	Text RAMA_48201_08061381520037_TURNITIN.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (8MB) | Request a copy
Preview	Text RAMA_48201_08061381520037_0003077102_0029128405_01_front_ref.pdf - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (779kB) | Preview
	Text RAMA_48201_08061381520037_0003077102_0029128405_02.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (621kB) | Request a copy
	Text RAMA_48201_08061381520037_0003077102_0029128405_03.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (148kB) | Request a copy
	Text RAMA_48201_08061381520037_0003077102_0029128405_04.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (218kB) | Request a copy
	Text RAMA_48201_08061381520037_0003077102_0029128405_05.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (6kB) | Request a copy
	Text RAMA_48201_08061381520037_0003077102_0029128405_06_ref.pdf - Bibliography Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (234kB) | Request a copy
	Text RAMA_48201_08061381520037_0003077102_0029128405_07_lamp.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy

Abstract

Wistar Strain Male Rats with Fixed-Dose-Procedure Method. Mona Riska Andhini 08061381520037 ABSTRACT Robusta coffee leaf (Coffea canehora) is one of the plants used by the community as antidiabetic. Aside from being an antidiabetic, other properties are antimicrobial and antioxidant. The results of chemical composition screening containing coffee leaf substances contain alkaloids, flavonoids, phenolics, tannins, terpenoids and steroids. Chemical compounds contained in powerful robusta coffee leaves need safety drugs that are supported by toxicity testing. In this research, the toxicity test of the ethanol extract of Robusta (Coffea canephora) ethanol extract on Wistar strain male rats was carried out using the fixed dose procedure method. The ethanol extract of Robusta coffee leaves was obtained by meseration using 96% ethanol. The initial dose chosen for the main test is determined from the results of the preliminary test. Based on preliminary test results, a dose of 2000 mg / kgBW was determined as the dose in the main test. In the main test, the test animals were divided into 2 groups, namely the normal group and the 2000 mg / kgBB dose group, each consisting of 5 test animals. The results of observations in the main test showed that in the normal group and the dose group of 2000 mg / kgBW there were no deaths or toxic effects in the test animals. The range of toxic dosages of Robusta coffee leaf extract is> 2000 mg / kgBB. Ethanol extract of Robusta coffee leaves did not fight macroscopic renal, liver and cardiac test animals (р> 0.05). The average level of biochemical parameters of the normal group was SGOT 102.83 ± 26.00 U / L, SGPT 88.15 ± 17.27 U / L, creatinine 0.67 ± 0.09 mg / dL and ureum 18.01 ± 2 , 24 mg / dL. Whereas the dose group of 2000 mg / kgBW were SGOT 114.50 ± 31.48 U / L, SGPT 106.65 ± 36.16 U / L, creatinine 1.20 ± 1.0 mg / dL and ureum 36.40 ± 3 .07 mg / dL. Keywords: Robusta coffee leaves, Coffea Canephora., Acute toxicity, fixed dose procedure. Indralaya, 03 Agustus 2020 Menyetujui Pembimbing 1 Pembimbing 2 Herlina, M.Kes., Apt. Annisa Amriani, S.M. Farm., Apt. NIP.197107031998022001 NIP.198412292014082201 Mengetahui, Ketua Jurusan Farmasi Dr.rer.nat. Mardiyanto, M.Si., Apt. NIP.197103101998021002 Uji Toksisitas Akut Ekstrak Etanol Daun Kopi Robusta (Coffea canephora) Terhadap Tikus Jantan Galur Wistar dengan Metode Fixed-Dose-Procedure. Mona Riska Andhini 08061381520037 ABSTRAK Daun kopi robusta (Coffea canehora) merupakan salah satu tanaman yang digunakan oleh masyarakat sebagai antidiabetes. Selain sebagai antidiabetes, khasiat lainnya adalah sebagai antimikroba dan antioksidan. Hasil skrining kandungan kimia menunjukkan bahwa daun kopi robusta mengandung senyawa berupa alkaloid, flavonoid, fenolik, tannin, terpenoid dan steroid. Senyawa kimia yang terkandung di dalam daun kopi robusta yang berkhasiat sebagai obat perlu diketahui keamanan penggunaannya yang didukung oleh pengujian toksisitas. Pada penelitian ini telah dilakukan pengujian toksisitas akut ekstrak etanol daun kopi robusta (Coffea canephora) terhadap tikus jantan galur wistar dengan metode fixed dose procedure. Ekstrak etanol daun kopi robusta diperoleh dengan cara meserasi menggunakan etanol 96%. Dosis awal yang dipilih untuk uji utama ditentukan dari hasil uji pendahuluan. Berdasarkan hasil uji pendahuluan, dosis 2000 mg/kgBB ditetapkan sebagai dosis pada uji utama. Pada uji utama hewan uji dibagi menjadi 2 kelompok yaitu kelompok normal dan kelompok dosis 2000 mg/kgBB yang masing-masing terdiri dari 5 ekor hewan uji. Hasil pengamatan pada uji utama menunjukkan bahwa pada kelompok normal dan kelompok dosis 2000 mg/kgBB tidak terjadi kematian maupun gejala toksis pada hewan uji. Rentang dosis toksis ekstrak daun kopi robusta adalah dosis >2000 mg/kgBB. Ekstrak etanol daun kopi robusta tidak berpengaruh terhadap makroskopis ginjal, hati dan jantung hewan uji (р>0,05). Rata-rata kadar parameter biokimia kelompok normal SGOT 102,83±26,00 U/L, SGPT 88,15±17,27 U/L, kreatinin 0,67±0,09 mg/dL dan ureum 18,01±2,24 mg/dL. Sedangkan kelompok dosis 2000 mg/kgBB adalah SGOT 114,50±31,48 U/L, SGPT 106,65±36,16 U/L, kreatinin 1,20±1,0 mg/dL dan ureum 36,40±3,07 mg/dL. Kata Kunci: Daun kopi robusta, Coffea Canephora., toksisitas akut, fixed dose procedure. Acute Toxicity Test of Robusta Coffee (Coffea canephora) Ethanol Extract on Wistar Strain Male Rats with Fixed-Dose-Procedure Method. Mona Riska Andhini 08061381520037 ABSTRACT Robusta coffee leaf (Coffea canehora) is one of the plants used by the community as antidiabetic. Aside from being an antidiabetic, other properties are antimicrobial and antioxidant. The results of chemical composition screening containing coffee leaf substances contain alkaloids, flavonoids, phenolics, tannins, terpenoids and steroids. Chemical compounds contained in powerful robusta coffee leaves need safety drugs that are supported by toxicity testing. In this research, the toxicity test of the ethanol extract of Robusta (Coffea canephora) ethanol extract on Wistar strain male rats was carried out using the fixed dose procedure method. The ethanol extract of Robusta coffee leaves was obtained by meseration using 96% ethanol. The initial dose chosen for the main test is determined from the results of the preliminary test. Based on preliminary test results, a dose of 2000 mg / kgBW was determined as the dose in the main test. In the main test, the test animals were divided into 2 groups, namely the normal group and the 2000 mg / kgBB dose group, each consisting of 5 test animals. The results of observations in the main test showed that in the normal group and the dose group of 2000 mg / kgBW there were no deaths or toxic effects in the test animals. The range of toxic dosages of Robusta coffee leaf extract is> 2000 mg / kgBB. Ethanol extract of Robusta coffee leaves did not fight macroscopic renal, liver and cardiac test animals (р> 0.05). The average level of biochemical parameters of the normal group was SGOT 102.83 ± 26.00 U / L, SGPT 88.15 ± 17.27 U / L, creatinine 0.67 ± 0.09 mg / dL and ureum 18.01 ± 2 , 24 mg / dL. Whereas the dose group of 2000 mg / kgBW were SGOT 114.50 ± 31.48 U / L, SGPT 106.65 ± 36.16 U / L, creatinine 1.20 ± 1.0 mg / dL and ureum 36.40 ± 3 .07 mg / dL. Keywords: Robusta coffee leaves, Coffea Canephora., Acute toxicity, fixed dose procedure.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Daun kopi robusta, Coffea Canephora., toksisitas akut, fixed dose procedure.
Subjects:	R Medicine > RS Pharmacy and materia medica > RS160-167 Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Divisions:	08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User:	Users 7423 not found.
Date Deposited:	18 Aug 2020 07:25
Last Modified:	18 Aug 2020 07:25
URI:	http://repository.unsri.ac.id/id/eprint/33165

Actions (login required)

View Item