Melki, Melki and Isnansetyo, Alim and Murwantoko, Murwantoko and Widada, Jaka (2018) The Significance of Water Quality Parameters on the Diversity of Ammonia-Oxidizing Bacteria in the Water Surface of Musi River, Indonesia. AACL Bioflux, 11 (6). pp. 1908-1918. ISSN 1844-8143
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Abstract
This study was conducted in Musi river area to assess the extent of ammonia-oxidizing bacteria (AOB) diversity as affected by land-use activities. PCR-Terminal Restriction Fragment Length Polymorphism (PCR-TRFLP) analysis of 16S rRNA gene was used to evaluate the diversity of AOB. A total of 158 Terminal Restriction Fragments (TRFs) of AOB in different sizes digested with BsuRI and MspI were obtained from all samples that were aligned to representative published sequences of 13 strains. The number of TRFs in each sample ranged from 52 to 491. There were eight strains of AOB results of digesting the two-restriction enzymes within the beta-subdivision ammonia-oxidizers namely Nitrosomonas europaea, itrosomonas eutropha, Nitrosomonas ureae, Nitrosomonas europaea strain C-31, Nitrosospira briensis, Nitrosospira briensis Nsp10, Nitrosococcus mobilis Nc2, and Nitrosolobus multiformis, its had the similarity coefficient in the range of 93-96%. The environmental conditions that affect the diversity of AOB are salinity, temperature, DO, and nutrients.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QR Microbiology > QR100-130 Microbial ecology |
| Divisions: | 08-Faculty of Mathematics and Natural Science > 54241-Marine Science (S1) |
| Depositing User: | Mr. Melki Melki |
| Date Deposited: | 31 Aug 2020 04:42 |
| Last Modified: | 31 Aug 2020 04:56 |
| URI: | http://repository.unsri.ac.id/id/eprint/33993 |
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