ISOLASI SENYAWA TURUNAN FLOROGLUSINOL DARI FRAKSI ETIL ASETAT BUAH KARAMUNTING (Rhodomyrtus tomentosa) DAN UJI PENGHAMBATAN AKTIVITAS ENZIM α-GLUKOSIDASE

ALFARADO, DANIEL and Ferlinahayati, Ferlinahayati and Eliza, Eliza (2019) ISOLASI SENYAWA TURUNAN FLOROGLUSINOL DARI FRAKSI ETIL ASETAT BUAH KARAMUNTING (Rhodomyrtus tomentosa) DAN UJI PENGHAMBATAN AKTIVITAS ENZIM α-GLUKOSIDASE. Undergraduate thesis, Sriwijaya University.

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Abstract

Diabetic mellitus (DM) is a disease with hyperglycemia condition. One of the DM treatment is to inhibit the α-glucosidase enzyme activity. Some of plant have been reported as inhibitor of α-glucosidase enzyme. Karamunting (Rhodomyrtus tomentosa) has been reported to have a variety of activities such as antioxidant, antibacterial, anticancer and antiinflamatory, however there is no report about theablility karamunting to inhibit the α-glucosidase enzyme activity. According to that the research was conducted to isolate the chemical compound and determine the ability of Karamunting plant as well as the isolate compound to inhibit the activity of α-glucosidase enzyme from extract, fraction and product of isolation. The extraction process was done by maceration using methanol, and then continued with liquid-liquid fractionation using n-hexane, ethyl acetate and n-butanol. Separation and purification of ethyl acetate fraction was conducted by vacuum liquid chromatography and radial chromatography. The isolate compound was a brownish white solid (19 mg) which decomposes at 85C. Based on spectrum analysis UV, IR, 1H-NMR, 13C-NMR, HSQC, HMBC and the comparison with the literature, it was concluded the isolated compound is phloroglucinol derivative namely isovaleroyl phloroglucinol. The IC50 of α-glucosidase enzyme activities from methanol extract, ethyl acetate fraction, n-butanol fraction and the isolate compound are 13,7; 9,04; 13,82; and 51,81 ppm respectively. Those extract and fractions have inhibition ability stronger than the reference inhibitor, acarbose (IC50 75,25 ppm). n-hexane fraction with IC50 value 727,96 ppm give weaker inhibition for α-glucosidase enzyme than acarbose. From this research, we can conclude that karamunting plant have an ability to inhibit the enzyme α-glucosidase activity.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Rhodomyrtus tomentosa, phloroglucinol derivative compound, isovaleroyl phloroglucinol, α-glukosidase enzymes
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Users 14 not found.
Date Deposited:	17 Jul 2019 07:56
Last Modified:	24 Jul 2019 14:54
URI:	http://repository.unsri.ac.id/id/eprint/374

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