EKSPRESI PROTEIN REKOMBINAN PEPTIDE YY (PYY) PADA Escherichia coli

ERNANTO, JUNORETTA HAVIVA and Amalia, Ella and Theodorus, Theodorus (2021) EKSPRESI PROTEIN REKOMBINAN PEPTIDE YY (PYY) PADA Escherichia coli. Undergraduate thesis, Sriwijaya University.

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Abstract

Background: PYY (1-36), a polypeptide consisting of 36 amino acid structures, is known to play a role in increasing the growth and survival of beta cells through NPY1R activation in the pancreas, which ultimately leads to glycemic balance. (P3L31P34)PYY(1-36) is a PYY (1-36) analog that is resistant to DPP-IV degradation and has high selectivity to NPY1R. This study aims to identify the characteristics of the (P3L31P34)PYY(1-36) recombinant protein-coding gene and its prediction of homologous proteins. Methods: This study is an experimental study with a computational approach. Data in the form of amino acid sequences were accessed from Pubmed and the iGEM registry. This study uses several website-based applications including OptimumGeneTM, Predict Protein, Protein-SOL, and SWISS-MODEL. Results: Construction 1 was characterized by a secondary structural composition consisting of α-helix and loop, and a solubility value of 0.5. While construction 2, the secondary structure composition contains α-helix, β-strand, and loop with a solubility value of 0.821. In the (P3L31P34)PYY(1-36) modelling, three homologous protein models were obtained. Conclusion: Construction 1 and 2 have different secondary structures. Meanwhile, the solubility value of construction 2 has a higher value. (P3L31P34)PYY(1-36) has three homologous protein models. Keywords: PYY (1-36), (P3L31P34)PYY(1-36), recombinant protein, computational approach

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	PYY (1-36), (P3L31P34)PYY(1-36), protein rekombinan, pendekatan komputasional
Subjects:	R Medicine > R Medicine (General) > R5-920 Medicine (General) R Medicine > RS Pharmacy and materia medica > RS441 Microscopical examination of drugs
Divisions:	04-Faculty of Medicine > 11201-Medicine (S1)
Depositing User:	Users 10100 not found.
Date Deposited:	20 Jan 2021 04:41
Last Modified:	20 Jan 2021 04:41
URI:	http://repository.unsri.ac.id/id/eprint/40634

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