ISOLASI METABOLIT SEKUNDER DARI JAMUR ENDOFITIK EKSTRASELULER DAUN MANGGIS (Garcinia mangostana) DAN AKTIVITAS ANTIOKSIDANNYA

PUTRI, AYU and Elfita, Elfita and Muharni, Muharni (2018) ISOLASI METABOLIT SEKUNDER DARI JAMUR ENDOFITIK EKSTRASELULER DAUN MANGGIS (Garcinia mangostana) DAN AKTIVITAS ANTIOKSIDANNYA. Undergraduate thesis, Sriwijaya University.

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Abstract

Six endophytic fungi from mangosteen leaf have been isolated and also screening antioxidant activity with DPPH method (1,1-Diphenyl-2-picryl-hydrazil). Extract endophytic fungi DM6 provides high antioxidant activity with IC50 13,21 μg / mL. Fungi DM6 proceeds to the isolation phase of its secondary metabolite. Endophytic fungi was cultivated in a 300 mL medium PDB (in 10 bottles), then incubated for 6 weeks at room temperature and in a static state. Extraction of extracellular secondary metabolite compounds was carried out partitionally, then evaporated and gravitational column chromatography. The results of pure compound isolation identified using UV, FT-IR, and NMR spectrophotometers. Structure of the compound endophytic fungi of mangosteen leaf is 6-(sec-butyl)-4-hydroxy-3-methyl-2H-pyran-2-one. Then, the pure compound isolation have antioxidant activity and gave IC50 461,13 μg / mL. These results suggest that the pure compound of endophytic fungi of mangosteen leaf is classified as inactive as an antioxidant because IC50> 100 μg /mL.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	endophytic fungi, mangosteen leaf (Garcinia mangostana), antioxidant
Subjects:	Q Science > QD Chemistry > QD1-999 Chemistry Q Science > QD Chemistry > QD241-441 Organic chemistry Q Science > QD Chemistry > QD701-731 Photochemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User:	Mrs Dies Meirita Sari
Date Deposited:	12 Aug 2019 04:10
Last Modified:	12 Aug 2019 04:10
URI:	http://repository.unsri.ac.id/id/eprint/4110

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