INDRIATI, WINDA and Yustian, Indra and Setiawan, Arum (2020) Quantitative and Qualitative Test of the Fecal Sampel From SumatranElephant (Elephas maximus sumatranus). BIOVALENTIA: Biological Research Journal, 6 (2). pp. 15-20. ISSN 24771392
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Abstract
Sumatran Elephants (Elephas maximus sumatranus) was one of the endemic Sumatran fauna that was protected by Indonesia Law. The Acacia Industrial Forest District Penyabungan in the landscape of Padang Sugihan was one of the elephant’s habitats in South Sumatra. Habitat degradation and fragmentation, illegal hunting and conflicts become the major threats for the elephant population. Genetic study was one of the efforts to establish a conservation policy. It was necessary to test the quantitative and qualitative of DNA, at the extractions stage from fecal samples, in order to get the standard results on DNA concentration which will be analyzed later. The method used in this study was direct observation as the initial survey to determine the sampling location. Fecal samples were preserved in absolute ethanol at -20°C. The extraction process was carried out using the Quick-DNATM Fecal/Soil Microbe Miniprep Kit. Samples were tested quantitatively using a Nanodrop Thermo Scientific, with the expectation that the concentration results were considered to be 1.6-1.8 and continued with the qualitative test of DNA through electrophoresis gel agarose and UV Transilluminator of the 20 samples/ There were only 12 samples that showed positive results (there were DNA bands). Theresul ts of this quantitative and qualitative test could be used to determine a viable sample to be used as a product in the genetic analysis stage of Sumatran Elephants in the Acacia Industrial Forest of South Sumatra.
| Item Type: | Article |
|---|---|
| Additional Information: | Jurnal Nasional Terakreditasi (Sinta 3) |
| Subjects: | Q Science > QL Zoology > QL700-739.8 Mammals |
| Depositing User: | Dr Arum Setiawan |
| Date Deposited: | 05 Feb 2021 09:08 |
| Last Modified: | 05 Feb 2021 09:08 |
| URI: | http://repository.unsri.ac.id/id/eprint/41775 |
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