SELEKSI PRIMER UNTUK PENDEKATAN MIKROSATELIT PADA AKSESI PADI BERAS MERAH DAN BERAS HITAM (Oryza sativa L.) LOKAL SUMATERA SELATAN

WAHYUNI, TUTIK and Hanum, Laila and Muharni, Muharni (2021) SELEKSI PRIMER UNTUK PENDEKATAN MIKROSATELIT PADA AKSESI PADI BERAS MERAH DAN BERAS HITAM (Oryza sativa L.) LOKAL SUMATERA SELATAN. Undergraduate thesis, Sriwijaya University.

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Abstract

Microsatelite markers or Simple Sequence Repeats (SSR) have been widely used for analysis of genetic identity determination in eukaryotes such as rice to overcome the threat of genetic erosion of local varieties. Microatelites have a large sequence of repetitions in the genome and spread evenly throughout the plant genome. However not all microsatelites match the sample used. Therefore, research is carried out that aims to determine microsatelite markers or Simple sequence Repeats (SSR) that are suitable for samples of brown rice and black rice locally in South Sumatra before further analysis. This research was conducted from July to October 2020. Sampling was conducted in several areas in South Sumatra. This research method begins with genome DNA isolation, DNA isolation results in quality and quantity, DNA amplification with PCR, and DNA amplification results using electrophoresis gel and observed under UV Transiluminator. The results of this study showed the presence of DNA tapes of different sizes. Nine of the eleven primary pairs of RM5742, RM6997, RM201, RM263, RM324, RM518, RM60, RM105, and RM124 had a high level of conformity to all samples. The two primers, primer RM416 is not suitable for Tanjung Agung and RM223 is not suitable for samples from Linggau so it is not able to amplify DNA in PCR process as a result of not forming DNA tape when electrophoresis is done. Keywords :Molecular markers, Microsatelite, SSR, DNA Isolation, PCR, Electrophoresis, Oryza sativa L.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Mikrosatelit, DNA Padi Beras Merah dan Beras Hitam
Subjects:	Q Science > Q Science (General) > Q1-295 General
Divisions:	08-Faculty of Mathematics and Natural Science > 46201-Biology (S1)
Depositing User:	Users 10887 not found.
Date Deposited:	05 Mar 2021 07:28
Last Modified:	05 Mar 2021 07:28
URI:	http://repository.unsri.ac.id/id/eprint/43507

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