DNA BARCODING IKAN BERINGIT (Mystus singaringan) ASAL SUNGAI BATANGHARI BERDASARKAN GEN SITOKROM C OKSIDASE SUBUNIT I (COI) = DNA BARCODING OF Mystus singaringan FROM BATANGHARI RIVER BASED ON CYTHOCROME C OXYDASE SUBUNIT I (COI) GENE

OCTRIANIE, NOER and Syaifudin, Mochamad and Jubaedah, Dade (2018) DNA BARCODING IKAN BERINGIT (Mystus singaringan) ASAL SUNGAI BATANGHARI BERDASARKAN GEN SITOKROM C OKSIDASE SUBUNIT I (COI) = DNA BARCODING OF Mystus singaringan FROM BATANGHARI RIVER BASED ON CYTHOCROME C OXYDASE SUBUNIT I (COI) GENE. Undergraduate thesis, Sriwijaya University.

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Abstract

Mystus singaringan is known as Bagrid catfish, one of species from the genus Mystus which often found in Batanghari river. This research aims to identify the sequence of Cythocrome C Oxydase Subunit I (COI) gene of mtDNA, construct the phylogenetic trees among species of M. singaringan and know the physical chemical of water characteristics of M. singaringan’s habitat in Batanghari river. The methods used in barcoding species and determining phylogenetic are DNA isolation, DNA amplification using PCR (Polymerase Chain Reaction) and sequencing COI gene regions of mtDNA. from the fish samples that were collected from Batanghari river, Jambi province. The COI gene were sequenced by PCR (Polymerase Chain Reaction) with optimum annealing temperature 50⁰C for 30s with 30 cycles. After having any editting, sequencing of the COI gene of Mystus singaringan produce 633 bp and 573 bp nucleotide length. Based on BLAST (Basic Local Alignment Search Tool) analysis compared to Genbank database, M. singaringan from Batanghari River had the higher similarity of 92% with M. singaringan from East Java and with the same species from Thailand as 86%. It’s claimed due to the existence of an ancient river about 17.000 to 20.000 years ago in the era of Pleistocene that connects between West Sumatera and East Java. The habitat characteristics of Mystus singaringan in Batanghari river were temperature 27.9-29.8oC, transparency 16.0-17.5 cm, dissolved oxygen 5.14-6.99 mgL-1, pH 7.8, ammonia 0.01-0.03 mgL-1 and total alkalinity 40 mgL-1 CaCO3.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Cythocrome C Oxydase Subunit I gene, M. singaringan, phylogenetic
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling > SH346-351 Fishery for individual species
Divisions:	05-Faculty of Agriculture > 54243-Aquaculture (S1)
Depositing User:	Users 12 not found.
Date Deposited:	19 Aug 2019 04:15
Last Modified:	19 Aug 2019 04:15
URI:	http://repository.unsri.ac.id/id/eprint/4517

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