PENGARUH KRIOPROTEKTAN DIMETIL SULFOKSIDA DOSIS BERBEDA DALAM EKSTENDER MADU TERHADAP KUALITAS SPERMA IKAN BELIDA (Chitala lopis) SELAMA MASA PENYIMPANAN

KUSUMA, M FERY ARTHA and Syaifudin, Mochamad and Tanbiyaskur, Tanbiyaskur (2021) PENGARUH KRIOPROTEKTAN DIMETIL SULFOKSIDA DOSIS BERBEDA DALAM EKSTENDER MADU TERHADAP KUALITAS SPERMA IKAN BELIDA (Chitala lopis) SELAMA MASA PENYIMPANAN. Undergraduate thesis, Sriwijaya University.

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Abstract

Cryoprotectant plays a role in saving biological material during cryopreservation. Dimethyl sulfoxide (DMSO) as a cryoprotectant has the ability to quickly penetrate into the cell at equilibration and leave the cell at thawing procedure. This study aims to determine the best dose of DSMO in honey extender to the characteristics of the featherback Fish spermatozoa cells in the cryopreservation process. This study used a completely randomized design (CRD) consisting of P0 control (0.5% honey in ringers solution) and three different doses of DMSO, namely P1 (0.5% honey in Ringer's solution + 10% DMSO), P2 (0.5% honey in Ringer's solution + 15% DMSO), and P3 (0.5% honey in Ringer's solution + 20% DMSO). The results showed that at storage time of 14 days, P3 gave the best results on motility (score 4), and the viability value was 77.44%, higher than Control, P1 and P2. In addition, the treatment did not show any abnormalities that occurred in the featherback fish sperm.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: dimetil sulfoksida, kriopreservasi, sperma ikan belida.
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling > SH151-179 Fish culture > SH155.6.M48 Fishes--Artificial spawning. Fishes--Germplasm resources--Cryopreservation. Shellfish--Artificial spawning.
S Agriculture > SH Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture
Divisions: 05-Faculty of Agriculture > 54243-Aquaculture (S1)
Depositing User: Users 11807 not found.
Date Deposited: 30 Apr 2021 01:37
Last Modified: 20 May 2021 06:48
URI: http://repository.unsri.ac.id/id/eprint/46004

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