ENKAPSULASI BAKTERI PROBIOTIK LACTOBACILLUS BULGARICUS MENGGUNAKAN ULTRA TURRAX DENGAN VARIASI KONSENTRASI NA-ALGINAT

FEBRIYANTI, ERISKA and Miksusanti, Miksusanti and Solihah, Indah (2021) ENKAPSULASI BAKTERI PROBIOTIK LACTOBACILLUS BULGARICUS MENGGUNAKAN ULTRA TURRAX DENGAN VARIASI KONSENTRASI NA-ALGINAT. Undergraduate thesis, Sriwijaya University.

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Abstract

Encapsulation of probiotic bacteria Lactobacillus bulgaricus used ultra turrax with variations in na-alginate concentration aimed to determine the optimal or best concentration of the probiotic bacteria formula L. bulgaricus against its viability and antibacterial activity, as well as the size of particle diameter, PDI, %EE, and zeta potential of the formed microparticles. Microparticles were made from the probiotic bacteria Lactobacillus bulgaricus by ultra turrax method by utilizing its antibacterial properties against pathogenic bacteria Escherichia coli. Variations in na-alginate concentrations are 1%, 2%, and 3%. The optimal concentration needed to obtain the optimal formula was 2%. The greater the concentration, the greater the imbalance between the carboxylic anion of Na-alginate and the cation of CaCl2 made it difficult to cross-bind with the CaCl2 cross linker which is only 40 μL. The smaller the concentration, the lower the viscosity and made it difficult to cross-bind with the CaCl2 crossed linker. Viability testing on all three consecutive formulas is 2.45 x 1015 cfu/mL, 2.78 x 1015 cfu/mL, 1.83 x 1015 cfu/mL. The characterization of the optimum microparticle formula had a diameter of 4688.75 nm, PDI of 0.691 and zeta potential of -47.8 mV. Antibacterial testing in formula II is 11 mm (strong category) while in positive control was 8.5 mm (medium category). Antibacterial testing of E. coli showed that the encapsulated probiotic bacteria L. bulgaricus had better antibacterial activity compared to the uncapsulated probiotic bacteria L. bulgaricus. The best formula stability was formula II which had a value of %EE of 85% in the first cycle and 73.7% in cycle 6. Keywords : Encapsulation, Lactobacillus bulgaricus, ultra turrax, antibacterial, na-alginate concentration variation

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Enkapsulasi, Lactobacillus bulgaricus, ultra turrax, antibakteri, variasi konsentrasi na-alginat.
Subjects:	Q Science > QR Microbiology > QR1-502 Microbiology Q Science > QR Microbiology > QR100-130 Microbial ecology Q Science > QR Microbiology > QR75-99.5 Bacteria R Medicine > RM Therapeutics. Pharmacology > RM265-267 Antibiotic therapy. Antibiotics R Medicine > RS Pharmacy and materia medica > RS192-199 Pharmaceutical technology
Divisions:	08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User:	Eriska Febriyanti
Date Deposited:	11 Jun 2021 08:04
Last Modified:	14 Jun 2021 03:45
URI:	http://repository.unsri.ac.id/id/eprint/47811

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