Antibacterial Effect Propolis Against Escherichia Coli and Shigella Dysenteriae In Vitro

Roni Ferdi, Irsan Saleh, Theodorus Theodorus, Salni Salni

Abstract


The bacteria that often cause infectious diarrhea are Escherichia coli and Shigella dysenteriae. Excessive and less rational use of antibiotics in diarrhea cases encourages the development of multi drug pathogen resistance. Propolis is one solution to overcome diarrheal infections. The purpose of this study was to determine the ability of the antibacterial effects of propolis extract on Escherichia coli and Shigella dysenteriae bacteria in in vitro testing with various concentrations. Research in laboratory experiments in vitro. The sample in this study was the bacteria Escherichia coli and Shigella dysenteriae with the stages of research starting from the extraction process carried out using a multilevel extraction method. The results showed that the smallest concentration of n-hexane fraction which still inhibited the growth of Escherichia coli and Shigella dysenteriae bacteria was 250 µg / ml and this concentration was expressed as KHM value. The equality test of 250 µg / ml n-hexane propolis fraction was equivalent to 4.0 µg / ml ciprofloxacin to Escherichia coli and equivalent to 4.6 µg / ml ciprofloxacin against Shigella dysenteriae, while the equality test concentration of 250 µg / ml ethyl acetate propolis equivalent to 5.2 µg / ml of ciprofloxacin against Escherichia coli and equivalent to 4.5 µg / ml of ciprofloxacin against Shigella dysenteriae. The conclusion of this study is that n-hexane extract and ethyl acetate propolis have antibacterial effectiveness against Escherichia coli and Shigella dysenteriae. The n-hexane and ethyl acetate propolis fractions have lower antibacterial activity compared to ciprofloxacin in inhibiting the growth of Escherichia coli and Shigella dysenteriae. Antibacterial compounds contained in the n-hexane fraction and ethyl acetate propolis are flavonoids and phenol 


Keywords


propolis, antibacteria, Escherichia coli, Shigella dysenteriae

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References


Adisasmito, W. 2007. Faktor Resiko Diare pada Bayi dan Balita di Indonesia.

Jurnal MAKARA 11 (1):1-10.

(http://www.jurnal.ui.ac.id)

Bankova, V. 2000. Determining Quality in Propolis Samples. (http://www. apitherapy.org/ determiningquality.html)

Brock, JM, Brock, K., John, F. 1996. Microbiology and Applications. Prentice Hall Inc. Englewood Cliffs. New Jersey.

Challem, J. 1995. Medical Journal Decument Value of Bee Propolis, Honey and Royal Jelly. http://www.thenutritionreporter.com/be e_stuff.html.

Ciesla WP, Guerrant RL. 2003. Infectious Diarrhea. In: Wilson WR, Drew WL, Henry NK, et al editors. Current

Diagnosis and Treatment in Infectious Disease. New York: Lange Medical Books 68:225.

Davis, W.W. And T.R. Stout.1971. Disc Plate Methods of Microbiological

Antibiotic Assay. Microbiology 22:659-

Departemen Kesehatan Provinsi Sumatera Selatan. 2010. Profil Kesehatan Propinsi Sumatera Selatan 2010 : “Distribusi Penderita Diare Semua Umur “, Sumsel.

Departemen Kesehatan Republik Indonesia. 2011. Profil Kesehatan Indonesia 2011 : “Penyakit Menular Berpotensi menimbulkan KLB”, Jakarta.

Harborne, J.B. 1987. Metode Fitokimia

Penuntun cara Modern Menganalisis Tumbuhan. Edisi Kedua. Terjemahan oleh : Padmawinata dan Soediro. ITB Bandung.

Hasan, A E Z. 2006. Potensi Propolis Lebah Madu Trigona spp. Sebagai Bahan Antibakteri. Seminar Nasional HKI:

Bogor.

Hill. R. 2000. Propolis The Natural

Antibiotic.

(www.Arkson.com/resources/ipropolis.ht m)

Hegazi, A.G. 1997. Propolis an Overview. J. Immunal. http: //www. apinetla. com.

ar/congreso/c05.pdf.

Holetz, F.B.H., Pessini, G.L., Sanches, N.R., Cortez, D.A.G., Nakamura, C.V., and Filho, B.P.D., 2002, Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. (http://www.biolineorg.br/request?oc02229).

Hyun Koo, Pedro L. Rosalen, Jaime A. Cury, Yong K. Park, and William H. Bowen. 2002. Effects of Compounds Found in Propolis on Streptococcus mutans Growth and on

Glucosyltranferase Activity. Center for Oral Biology and Eastman Departement of Dentistry, University of Rochester Medical Center, Rochester, New York.

Hal 1-2

Lenny, S. 2006. Senyawa Flavonoida, Fenil Propanoida dan Alkaloid. USU.

Medan.

Leaven, M., Berghe, F.,Marters, A., Vlientinck,E., Lammens. 1999. Screening of Hinger Plants for Biologycal Activity.

Planta Medica. New York.

Lung E, Acute Diarrheal Disease. In: Friedman SL, McQuaid KR, Grendell JH, editors. Current Diagnosis and Treatment

nd in Gastroenterology. 2 edition. New

York: Lange Medical Books, 2003. 131 -

Panzani, S.W. 2009. An Examination of Antibacterial and Antifungal Properties of Constituents described in Traditional Ulster Cures and Remedies. The ulster medicinal society. 78(1): 13-15.

Pelczar, M.J. & E.C.S. Chan. 2005. Dasardasar Mikrobiologi. Jilid II. Terjemahan oleh : Hadioetomo, R.S., Tjitrosomo, S.S., Angka, S.L. & Imas, T. UI Press. Jakarta.

Poliklinik RSMH. 2009. Laporan Kejadian Diare akut di poli anak umum.

Palembang, hal 1-68.

Prescot, 2005. Microbiology. 6nd Edition. Mc. Graw Hill Companies. Inc. New York. xxi +992 hlm.

Prindle, R,F. 1993. Phenolic Compounds. In Block S.S. Ed. Disinfection Sterilization and Preservation. Philadelphia: Lea and Fibiger.

Salatino A, Teixeira ÉW, Negri G, Message D. 2005. Origin and chemical variation of Brazilian propolis. eCAM 2:33–38.

Salni. 2003. Karakterisasi dan Uji Aktivitas Topikal Senyawa Antibakteri dari Daun Karamunting {Rhodormyrtus tomentosa (Ait) Hassk} dan Uji Efektivias Sediaan Salepnya. Disertasi. ITB Bandung. 153 hlm.

Saputra I. 2009. Aktivitas antibakteri mikrokapsulasi propolis Trigona spp. Pandeglang setelah terpapar cairan lumen sapi [skripsi]. Bogor: Fakultas Matematika dan Ilmu Pengetahuan

Alam. Institut Pertanian Bogor.

Sforcin, J.M, Funari, S.R.C, Novelli, E.L.B. 1995. Serum biochemical determinations of propolis-treated rats. J Venom. Anim.

Toxins Vol. 1

Suharto. 2002 . Mikrobiologi Kedokteran Edisi Revisi : Flora Normal serta Hubungan Kuman Dengan Hospes dan Lingkungannya. Binarupa Aksara, Jakarta, Indonesia, hal 27-32.

Tadda A. 2010. Patofisiologi Gejala Klinik dan Penatalaksanaan Diare. [terhubung berkala] http://astaqauliyah.com/2010/06/artikel -kedokteranpatofisiologi- gejala-klinikdan-penatalaksanaan-diare (5 Januari 2013).

Winingsih, W. 2004. Kediaman Lebah Sebagai Antibiotik dan Antikanker.

http://www.Pikiran rakyat.com/cetak/0904/16/cakrawala/6.

htm




DOI: https://doi.org/10.32539/bji.v5i2.7982

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