UJI HAYATI FILTRAT BIAKAN JAMUR ENTOMOPATOGEN YANG DIINKUBASIKAN PADA SUHU 25 ºC DAN SUHU 34 ºC TERHADAP LARVA Spodoptera litura (FABRICIUS) (LEPIDOPTERA: NOCTUIDAE) PADA CABAI (Capsicum annuum L.) = BIOASSAY OF THE FILTRATE OF ENTOMOPATHOGENIC FUNGAL CULTURE INCUBATED UNDER TEMPERATURE 25 ºC AND 34 ºC AGAINST LARVAE OF Spodoptera litura (FABRICIUS) (LEPIDOPTERA: NOCTUIDAE) ON CHILLI (Capsicum annuum L.)

FAJRIAH, ANNISA JACQUELINE and Herlinda, Siti (2018) UJI HAYATI FILTRAT BIAKAN JAMUR ENTOMOPATOGEN YANG DIINKUBASIKAN PADA SUHU 25 ºC DAN SUHU 34 ºC TERHADAP LARVA Spodoptera litura (FABRICIUS) (LEPIDOPTERA: NOCTUIDAE) PADA CABAI (Capsicum annuum L.) = BIOASSAY OF THE FILTRATE OF ENTOMOPATHOGENIC FUNGAL CULTURE INCUBATED UNDER TEMPERATURE 25 ºC AND 34 ºC AGAINST LARVAE OF Spodoptera litura (FABRICIUS) (LEPIDOPTERA: NOCTUIDAE) ON CHILLI (Capsicum annuum L.). Undergraduate thesis, Sriwijaya University.

[thumbnail of RAMA_ 54211_ 05071181520020_ 0020106504_01_front_ref.pdf]
Preview
 Text
RAMA_ 54211_ 05071181520020_ 0020106504_01_front_ref.pdf - Accepted Version
Available under License Creative Commons Public Domain Dedication.
Download (552kB) | Preview
[thumbnail of RAMA_ 54211_ 05071181520020_ 0020106504_02.pdf] Text
RAMA_ 54211_ 05071181520020_ 0020106504_02.pdf - Accepted Version
Restricted to Repository staff only
Available under License Creative Commons Public Domain Dedication.
Download (355kB) | Request a copy
[thumbnail of RAMA_ 54211_ 05071181520020_ 0020106504_03.pdf] Text
RAMA_ 54211_ 05071181520020_ 0020106504_03.pdf - Accepted Version
Restricted to Repository staff only
Available under License Creative Commons Public Domain Dedication.
Download (1MB) | Request a copy
[thumbnail of RAMA_ 54211_ 05071181520020_ 0020106504_04.pdf] Text
RAMA_ 54211_ 05071181520020_ 0020106504_04.pdf - Accepted Version
Restricted to Repository staff only
Available under License Creative Commons Public Domain Dedication.
Download (1MB) | Request a copy
[thumbnail of RAMA_ 54211_ 05071181520020_ 0020106504_05.pdf] Text
RAMA_ 54211_ 05071181520020_ 0020106504_05.pdf - Accepted Version
Restricted to Repository staff only
Available under License Creative Commons Public Domain Dedication.
Download (86kB) | Request a copy
[thumbnail of RAMA_ 54211_ 05071181520020_ 0020106504_06.ref.pdf] Text
RAMA_ 54211_ 05071181520020_ 0020106504_06.ref.pdf - Bibliography
Restricted to Repository staff only
Available under License Creative Commons Public Domain Dedication.
Download (171kB) | Request a copy
[thumbnail of RAMA_ 54211_ 05071181520020_ 0020106504_07_lamp.pdf] Text
RAMA_ 54211_ 05071181520020_ 0020106504_07_lamp.pdf - Accepted Version
Restricted to Repository staff only
Available under License Creative Commons Public Domain Dedication.
Download (726kB) | Request a copy

Abstract

Spodoptera litura is one of important pest of chilli on which the insect can cause damage up to 40%. Entomopathogenic fungi such Beauveria bassiana and Metarhizium anisopliae have been widely used to control S. Litura. Entomopathognic fungi is microorganism usually used as biological control agent producing endotoxin and toxic against insect. There fore, the objective of this research were: 1) knowing the effect of culture filtrate of Beauveria bassiana incubated under temperature 25 ºC and 34 ºC on the mortality and lethal time of Spodoptera litura larvae, 2) knowing the effect of culture filtrate of Metarhizium anisopliae incubated under temperature 25 ºC and 34 ºC on the mortality and lethal time of Spodoptera litura larvae. The experiment was arranged in a Completely Randomized Block Design (CRBD) with 2 factors, the first factor was temperature and the second was fungal isolate. Entomopathogenic fungi was incubated under room temperature for 5 weeks then incubated under 25 ºC and 34 ºC for 7 days, depended on the treatment. The isolates used were 20 isolates consisting of 10 isolates of Beuveria bassiana and 10 isolates of Metarhizium anisopliae. The insect used was second instar of Spodoptera litura, 25 larvae per replicate. Data collected was analyzed using ANOVA. Individual mortality recorded daily was used to determined LT50 using probit analyses. The results showed that the highest mortality caused by isolate B. bassiana was found on the fungus incubated under 25 ºC and was significantly different from that of temperature 34 ºC. There was an interaction between the first and second factor, means that certain isolate was affected by certain temperature. For isolate factor (second factor), the highest mortality on isolate BTmPc (96%) incubated under temperature 25 oC, and was not significantly different from isolate Btmtr (93.33%), Blepd (90.57%), BTmkt (90.67%) temperature 25 ºC,. The significant different was found between isolate BtmGa (74.67%), isolate BMkMs (80.00% ), BPCmS (76.00%), BTmTs (61.00%), Ts1d3 (54.67%), Ts1d2 (61.33%) temperature 25 ºC, BTmPc (9.33%), BtmGa (8.00%), Blepd (12.00%), BMkMs (18.67%), BPCmS (14.67%), BTmkt (18.67%), BTmTr (14.67%), BTmTs ( 14.67%), Ts1d3 (14.67%), Ts1d2 (18.67%) temperature 34 ºC Under treatment of M. anisopliae, the highest mortality was found on the fungus incubated under temperature 25 ºC which was significant different from those of temperature 34 ºC, but no interaction between first and second factor. The shortest LT50 of larvae S. litura dropped with culture filtrate of B. bassiana was found on the fungus incubated under temperature 25 ºC and significantly different from that of temperature 34 ºC. There was no interaction between the first and second factors. The shortest LT50 of larvae S. litura dropped with culture filtrate of M. anisopliae was found on the fungus incubated under temperature 25 ºC and significantly different from that of temperature 34 ºC. There was no interaction between the first and second factors. For B. bassiana, the highest mortality was found on isolate BTmPc incubated under temperature 25 ºC with shortest LT50 (7.51 days), which was not significantly different from isolate BTmTr incubated under temperature 25 ºC with LT50 (7.31 days). Therefore, isolate BTmTr incubated under temperature 25 ºC was the most effective in killing larvae of S. litura. For M. anisopliae the highest mortality was found on isolate MPdB incubated under temperature 25 ºC with shortest LT50 (8.34 days), which was not significantly different from isolate MSwTp2 incubated under temperature 25 ºC with LT50 (8.16 days). Therefore, isolate MSwTp2 incubated under temperature 25 ºC was the most effective in killing larvae of S. litura.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Beauveria bassiana, Metarhizium anisopliae, Spodoptera litura, Temperature
Subjects: S Agriculture > SB Plant culture > SB621-795 Plant pathology
Divisions: 05-Faculty of Agriculture > 54211-Agricultural Technology (S1)
Depositing User: Users 12 not found.
Date Deposited: 27 Aug 2019 07:47
Last Modified: 27 Aug 2019 07:47
URI: http://repository.unsri.ac.id/id/eprint/4899

Actions (login required)

View Item View Item