Hermansyah, Hermansyah and Adhiyanti, Nurmalina and Julinar, Julinar and Rahadiyanto, Kemas Yakub and Susilawati, Susilawati (2017) IDENTIFICATION OF CANDIDA SPECIES BY ASSIMILATION AND MULTIPLEX-PCR METHODS. Journal of Chemical Technology and Metallurgy, 52 (6). pp. 1070-1078. ISSN 13147471/13147978
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Abstract
The species of Candida, responsible for causing candidiasis infection, require fast and accurate identi cation, so that drugs can be delivered right. A total of 16 glycerol stock samples, obtained from the woman’s prison at Palembang, were cultivated and rejuvenated on a Sabouraud Dextrose Agar (SDA) medium and identi ed by using the API 20C AUX kit. The results have identi ed C. glabrata, C. crusei, C. parapsilosis, C. albicans, C. Tropicalis; two samples have not been clearly identi ed. The result from the API 20C AUX kit method were then compared to the results from the fermentation method in a previous study. Samples which were not t or not identi ed were reanalyzed by using Multiplex-PCR. Prior to performing Multiplex PCR, DNA of Candida samples were isolated by using the heating method and the presence of DNA was con rmed by using a spectrophotometer. Primer pairs used in the Multiplex–PCR method were the universal primer ITS1-ITS2, and the speci c primers CA3 and CA4. The results identi ed C. tropicalis, C. glabrata, and C. parapsilosis about 218, 483, and 229 bp, respectively The match of both methods (the assimilation method, using the API 20C AUX Kit and the Multiplex-PCR method) was 70 %, which can support the use of assimilation methods for the identi cation of Candida species. The assimilation method using API 20C AUX Kit needs to spend about 2 days for identi cation, while the conventional method or a fermentation method needs 14 days.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QD Chemistry > QD415-436 Biochemistry |
| Depositing User: | Hermansyah Hermansyah |
| Date Deposited: | 17 Sep 2021 14:53 |
| Last Modified: | 17 Sep 2021 14:53 |
| URI: | http://repository.unsri.ac.id/id/eprint/53880 |
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