Growth Retardation of Saccharomyces cerevisiae by Noni Fruit (Morinda citrifolia) Extract Occurred in G1 to S Transition of the Cell Cycle

Hermansyah, Hermansyah and Susilawati, Susilawati and Subositi, Dyah Growth Retardation of Saccharomyces cerevisiae by Noni Fruit (Morinda citrifolia) Extract Occurred in G1 to S Transition of the Cell Cycle. JPCS. ISSN 17426588

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Abstract

: We previously reported that methanolic extract of noni fruit (Morinda citrifolia) can inhibit growth of Saccharomyces cerevisiae. This finding suggested that noni fruit has anti�proliferative effect. Previous data based upon FACS (Fluorescence Activated Cell Sorting) showed that growth of S.cerevisiae inhibited in G1 to S transition phase in the presence of 1% methanolic extract of noni fruit. Further analysis to explore genes involved in this slow transition step, we conducted real time PCR. Expression some genes changed in the presence of 1% methanolic extract of noni fruit. Transcriptional level of gene CDC28 (cyclin dependent kinase�28), CLN2, CLN3, dan SWI6 changed 2.25, 2.79, 4.87 and 7.21 folds, respectively. This data suggested that over transcription of those gene inhibit cell cycle progression. CDC28 is master regulator of mitotic and meiotic cell cycles, CLN2 and CLN3 are G1 cyclin which play an important role in regulation of cell cycle, and SWI6 is transcription cofactor where forms complexes with SWI4 and MBP1 in regulation of transcription at G1 to S phase transition

Item Type:	Article
Subjects:	Q Science > QD Chemistry > QD415-436 Biochemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Hermansyah Hermansyah
Date Deposited:	26 Sep 2021 07:14
Last Modified:	26 Sep 2021 07:14
URI:	http://repository.unsri.ac.id/id/eprint/54924

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