Miksusanti, Miksusanti and SAPUTRA, HARIAN and Sandi, Sofia and Hermansyah, Hermansyah (2016) The Effect of Lactobacillus acidophilus and hito−Oligosaccharide on Antibacterial Activity and Organic Acid Production. Indonesian Journal of Fundamental and Applied Chemistry. ISSN e-ISSN: 2540 9409 p-ISSN: 2540 9395
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Abstract
The effect of Lactobacillus acidophilus combined with and without Chito-Oligosaccharide (COS) on the growth of Escherichia coli and Staphylococcus aureus had been studied. The antibacterial activity of L. acidophilus before and after combination with COS 0.2% was tested on bacteria of E. coli and S. aureus with well-diffusion method. Incubation time was carried out in 44, 46, 48, 50, and 52 hours. Organic acids produced by L. acidophilus was measured by HPLC. The result showed that COS 0.2% can inhibit the growth of E. coli and S. aureus 37.2 mm2 and 52 mm2 respectively. Combination of L. acidophilus and COS 0.2% gave inhibition zone larger than L. acidophilus without COS 0.2%. Incubation time within 48 hours of L. acidophilus combined with COS 0.2% produced the largest inhibition zone against E. coli and S. aureus 367.92 mm2 and 343.99 mm2 respectively. Optical density measurement resulted in higher value for combination one but concentration of organic acid produced was lower compare to L. acidophilus without COS 0.2%
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QD Chemistry > QD1-999 Chemistry Q Science > QD Chemistry > QD415-436 Biochemistry |
| Divisions: | 08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1) |
| Depositing User: | Hermansyah Hermansyah |
| Date Deposited: | 26 Sep 2021 06:40 |
| Last Modified: | 26 Sep 2021 06:47 |
| URI: | http://repository.unsri.ac.id/id/eprint/54952 |
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