Mustopa, A Zaenal and Umami, Rifqiah Nur and Melki, Melki (2015) ANTIBACTERIAL ACTIVITY ASSAY OF MANGROVE EXTRACTS AGAINST SALMONELLA TYPHI AND LISTERIA MONOCYTOGENES (Peer Review 1&2). Jurnal Ilmu dan Teknologi Kelautan Tropis, 7 (2). pp. 603-612. ISSN 2476-9347
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Abstract
The antibacterial activities of mangrove species, Avicennia marina, Sonneratia caseolaris (collected from Teluk Payo, Banyuasin, South Sumatera), Ceriops tagal, Rizhopora apiculata, and Sonneratia alba (collected from Sadai, South Bangka) were screened against Salmonella typhi and Listeria monocytogenes by agar disk diffusion assays. Extractions were conducted using organic solvents (methanol, ethyl acetate, and acetone, subsequently). Most of the extracts tested showed potential antibacterial activity against both pathogens. The methanol extracts of the bark from S. alba and the fruit from A. marina showed particularly large inhibition zones (15 mm) against S. typhi. The acetone extract of S. alba leaves showed the largest inhibition zone (14 mm) when tested against L. Monocytogenes. Further partial purifications of selected extracts which showed strong inhibition were performed by silica gel column chromatography using various eluent compositions with different polarities. The third fraction of methanol extract from S.alba leaves eluted with chloroform:methanol (1:5) resulted in a remarkably large inhibition zone (23 mm) against S. typhi. The third and seventh fractions of acetone extract from S. alba leaves eluted with ethyl acetate:methanol (7:3) resulted in a large inhibition zones (15 mm) against L. monocytogenes. In addition, the sixth fraction of methanol extract from A. marina fruit eluted with chloroform : methanol (6:4) resulted in the largest inhibition zone (17 mm) against L. monocytogenes. These results indicated that mangrove extracts could be developed as potential biomaterials for biopharmaceutical as well as biopreservation industries.
| Item Type: | Article |
|---|---|
| Subjects: | |
| Divisions: | 08-Faculty of Mathematics and Natural Science > 54241-Marine Science (S1) |
| Depositing User: | Dr Melki melki |
| Date Deposited: | 18 Jan 2022 06:12 |
| Last Modified: | 18 Jan 2022 06:12 |
| URI: | http://repository.unsri.ac.id/id/eprint/61032 |
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- ANTIBACTERIAL ACTIVITY ASSAY OF MANGROVE EXTRACTS AGAINST SALMONELLA TYPHI AND LISTERIA MONOCYTOGENES (Peer Review 1&2). (deposited 18 Jan 2022 06:12) [Currently Displayed]
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