MAWADDAH, HAFIZHA and Herlinda, Siti (2021) POTENSI JAMUR ENTOMOPATOGEN ASAL SERANGGA DARI SUMATERA SELATAN SEBAGAI JAMUR ENDOFIT PADA BIBIT JAGUNG DAN PATOGENISITASNYA TERHADAP LARVA Spodoptera frugiperda. Undergraduate thesis, Sriwijaya University.
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Abstract
Spodoptera frugiperda (FAW; Fall Armyworm J. E Smith, Lepidoptera: Noctuidae) is an important pest on maize that was first reported to be discovered in Indonesia in 2019. The emergence of this pest can pose a serious threat to Indonesia’s agricultural and food sectors because S. frugiperda is classified as an invasive pest. The use of entomopathogenic endophytic fungi is more effective because these fungi can enter plant tissues and can stimulate plant growth. This study aimed to determine the level of pathogenicity of endophytic entomopathogenic fungi derived from infected larvae in various maize fields in South Sumatra. This study was carried out with two experiments designed according to a Completely Randomized Design (CRD) with 11 isolates namely JGTP240521A, JGTP240521B, JGTS240521, JGMA260521, JGTB280521A, JGTB280521B, JGSR300521, JGCJ300521A, JGCJ300521B, JGND300521, JGNT310521 and a control (sterile water) treatment and repeated three times. The method used in this study, namely the test of fungal colonization on maize leaves and the test of potential entomopathogenic fungi that were proven to be endophytic against S. frugiperda larvae. The variables observed in this study included conidia density, conidia viability, leaf area eaten, larval body weight, larval manure weight, larval mortality, LT50, LT95, percentage of pupae appearing, normal and abnormal pupae, weight and length of pupae, number of adults appearing, normal and abnormal adult, body length and wing span of adult, lifespan of adult, number of eggs laid, number of hatching larvae, number of unhatched larvae, and the effect of entomopathogenic fungi on the growth of maize seedlings. The results showed that 5 isolates were proven to be endophytic, namely JGTP240521A, JGTB280521B, JGSR300521, JGCJ300521A, and JGNT310521 the highest percentage of fungi colonization 66.67%. The results showed that the highest spore density, the highest percentage of viability, and the highest mortality rate were obtained from larvae treated with the JGNT310521 isolate with a mortality percentage of 34.67% and the shortest LT₅₀ and LT₉₅, namely 13.33 days and 25.60 days. Eggs produced and the lowest egg numbers were obtained from the JGSR300521 treatment with an average number of 75.67 eggs and 67.67 hatching larvae and the highest was in the control treatment, with an average of 138.33 eggs. As for the agronomic variables test of maize seedlings aged 10 days after planting, the seeds that were applied with fungi were proven to have better growth and were significantly different from Control, with the highest plant growth on various variables obtained from the JGTP240521A treatment, and the lowest was in maize seedlings treated with entomopathogenic fungal isolates with code JGTP240521B. Conclusion of this study that 5 isolates were proven to be endophytic, namely JGTP240521A, JGTB280521B, JGSR300521, JGCJ300521A, and JGNT310521 the highest percentage of fungi colonization 66.67%. The highest mortality obtained in entomopathogenic fungi that proven to be endophytic were code JGNT310521 with a mortality percentage of 34.67% and the shortest LT₅₀ and LT₉₅, namely 13.33 days and 25.60 days.
Item Type: | Thesis (Undergraduate) |
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Uncontrolled Keywords: | Spodoptera frugiperda, jamur entomopatogen, jamur endofit |
Subjects: | S Agriculture > S Agriculture (General) > S1-(972) Agriculture (General) |
Divisions: | 05-Faculty of Agriculture > 54295-Plant Protection (S1) |
Depositing User: | HAFIZHA MAWADDAH |
Date Deposited: | 19 Jan 2022 07:47 |
Last Modified: | 19 Jan 2022 07:47 |
URI: | http://repository.unsri.ac.id/id/eprint/61924 |
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