JAMUR ENTOMOPATOGEN YANG BERSIFAT ENDOFIT DAN NON ENDOFIT ASAL SERANGGA DARI SUMATERA SELATAN PADA JAGUNG DAN PATOGENISITASNYA TERHADAP LARVA Spodoptera frugiperda

GUMAI, DYA ANASTASYA and Herlinda, Siti (2021) JAMUR ENTOMOPATOGEN YANG BERSIFAT ENDOFIT DAN NON ENDOFIT ASAL SERANGGA DARI SUMATERA SELATAN PADA JAGUNG DAN PATOGENISITASNYA TERHADAP LARVA Spodoptera frugiperda. Undergraduate thesis, Sriwijaya University.

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Abstract

Spodoptera frugiperda is an important pest of maize that can cause damage to crops and even death. The major damage of the plant causes the death of maize plants. S. frugiperda larvae were active in attacking almost all parts of the plant and all plant stages. Endophytic fungi control is more effective because the fungi can enter plant tissues. The purpose of this study was to determine the endophytic entomopathogenic fungi and to test the pathogenicity of endophytic entomopathogenic fungi against S. frugiperda larvae. In this study, the isolats used were isolats from insects found in South Sumatra. This study was carried out with two experiments designed according to a completely randomized design (CRD) with the treatment of 11 isolats with codes JGTP240521A, JGTP240521B, JGTS240521, JGMA260521, JGTB280521A, JGTB280521B, JGSR300521, JGCJ300521A, JGCJ300521B, JGND3005211, and control JGNT3105211, respectively. three times. The methods used in this study, namely the test of fungal colonization on maize leaves and the test of potential entomopathogenic fungi that were proven to be endophytic against larvae S. frugiperda larvae. The variables observed in this study included conidia density, conidia viability, leaf area eaten, larval body weight, larval droppings weight, larval mortality, LT50, LT95, percentage of pupae appeared, normal and abnormal pupae, weight and length of pupae, number of imago appeared, normal and abnormal imago, body length and wing span of imago, life span of imago, number of eggs laid, number of hatching larvae, number of unhatched larvae, and the effect of entomopathogenic fungi on the growth of maize seedlings. The results found 5 isolats of entomopathogenic fungi that have been tested for confirmation and colonization of fungi on corn leaves, namely JGTP240521A, JGTB280521B, JGSR300521, JGCJ300521A, and JGNT310521. Based on the appearance of macroscopic and microscopic structures of endophytic entomopathogenic fungi that are from the genus Beauveria sp. The highest percentage of endophytic fungal colonization on maize leaves was found in isolat JGNT310521, which was 73.33%. The highest mortality was found in isolat JGNT310521 with mortality reaching 30.66% and LT50 and LT95, namely 19.06 days and 38.32 days, respectively. The lowest percentage of pupae and imago that appeared was in isolat JGNT310521, namely 46.67% and 38.67%, respectively. The male and female life span of S. frugiperda imago was lowest found in isolat JGSR300521 with an average of 3 days. The number of S. frugiperda eggs lowestand imago appeared in isolat JGSR300521, namely 72.67 and 55.67. The conclusion of this study was the discovery of 5 isolats of endophytic entomopathogenic fungi from South Sumatra, namely JGTP240521A, JGTB280521B, JGSR300521, JGCJ300521A, and JGNT310521. The highest percentage of endophytic fungal colonization on maize seed was obtained from isolat JGNT310521. The endophytic entomopathogenic fungus isolat that had the highest pathogenicity in controlling S. frugiperda larvae was isolat JGNT310521.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Endophytic fungi, Entomopathogenic fungi, Spodoptera frugiperda
Subjects:	S Agriculture > S Agriculture (General) > S1-(972) Agriculture (General)
Divisions:	05-Faculty of Agriculture > 54295-Plant Protection (S1)
Depositing User:	Dya Anastasya Gumai
Date Deposited:	26 Jan 2022 07:15
Last Modified:	25 Apr 2022 06:56
URI:	http://repository.unsri.ac.id/id/eprint/62968

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