NANOPOLIMERIK KITOSAN-ALGINAT PEMBAWA EKSTRAK WHITE TEA (Camellia sinensis) SEBAGAI PENGHAMBAT STRES OKSIDATIF PADA TIKUS DIABETES TIPE 2

SHIYAN, SHAUM (2020) NANOPOLIMERIK KITOSAN-ALGINAT PEMBAWA EKSTRAK WHITE TEA (Camellia sinensis) SEBAGAI PENGHAMBAT STRES OKSIDATIF PADA TIKUS DIABETES TIPE 2. Doctoral thesis, Universitas Gadjah Mada.

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Abstract

The epigallocatechin gallate (EGCG) in white tea has unstable properties and poor absorption in the body. Drug carrier formulation needs to be done to improve its stability and effectiveness as an antioxidant in inhibiting oxidative stress. The hope is that it can obtain better biological or pharmacological activity. The extraction process using the infundation method is modified into kinetic-assisted infundation (KAI) to obtain an extract with abundant EGCG content. Extract quantification includes the determination of total phenolic and total flavonoid (spectrophotometric method) and EGCG levels (HPLC method). The nano polymeric formulation of white tea extract carrier uses the ionic gelation method with variations of chitosan and alginate. Formula optimization uses the design of experiment (DoE) approach. The characterization of nanopolymers includes particle morphology, particle evaluation, a study of material component interactions. Optimum nanopolymers and free extracts were carried out in vivo bioactivity testing on oxidative stress modeling with type 2 diabetes test animals. Test animals were induced by streptozotocin-nicotinamide (STZ-NA) by monitoring blood glucose levels. Oxidative stress parameters measured included superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GPx). Pancreatic and liver organs are made histological preparations with hematoxylin-eosin (HE) staining. The extraction method using the KAI technique can produce white tea extract with good characteristics. The optimal extraction conditions in the KAI technique can increase the yield, EGCG, TPC, and TFC content with the optimal factor at a concentration of 14.75%, stirring speed 440 rpm, and extraction time of 12.32 minutes. The optimum mixture of 0.11% chitosan, 0.04% alginate, and 0.06% extract produces a nano polymer with a particle size of 294.3 ± 6.98, a large particle ionic charge of 29.7 ± 1.31, and an EGCG absorption efficiency of 77,21 ± 0.80. Administration of white tea extract nanopolymers has been shown to suppress oxidative stress in type 2 diabetes using mice that were previously induced using STZ-NA. The overall results of this research can be used in the design of nutraceutical products that can help diabetes patients.

Item Type:	Thesis (Doctoral)
Subjects:	R Medicine > RS Pharmacy and materia medica > RS160-167 Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Divisions:	08-Faculty of Mathematics and Natural Science > 44001-Mathematics and Natural Science (S3)
Depositing User:	Shaum Shiyan
Date Deposited:	24 Feb 2022 04:02
Last Modified:	24 Feb 2022 04:02
URI:	http://repository.unsri.ac.id/id/eprint/64934

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