PENGARUH ENKAPSULASI DENGAN MALTODEKSTRIN TERHADAP PERTUMBUHAN DAN AKTIVTAS ANTIBAKTERI Streptococcus lactis sp

YOHANA, FANI and Miksusanti, Miksusanti and Kusumawati, Heni Yohandini (2014) PENGARUH ENKAPSULASI DENGAN MALTODEKSTRIN TERHADAP PERTUMBUHAN DAN AKTIVTAS ANTIBAKTERI Streptococcus lactis sp. Undergraduate thesis, Sriwijaya University.

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Abstract

Streptococcus lactis sp had been encapsulated with maItodextrin using spray drying method. Effect of pH, bile salt and sodium chlorida to the growth of with and without encapsulated of S. lactis sp was analyzed by Optical Density (OD). Organic acid that excreted by with and without encapsulated S. lactis sp was separated by chromatography technique using HPLC and the composition of organic acid was identified using organic acid Standard. Antibacterial activity was carried out by well diffusion method. 500 mL of suspention S. lactis sp in MRS Broth resulted 300 gram encapsulated bacteria with maltodextrin. The water content of encapsulated S. lactis sp was 11,27%. The endurance test against bile salt, pH and NaCl showed encapsulated tend better than without encapsulated bacteria. The optimum growth of S. lactis sp were at 0,2% bile salt, 2% natrium chlorida and at pH 6. The antibacterial of encapsulated more active than without encapsulated bacteria against E. coli and Salmonella typhimurium (positive Gram bacteria) and against S. aureus and B. cereus (negative Gram bacteria). The identification result of organic acid composition of the two treatments showed that the main composition of the organic acid excreted by S. lactis sp with and without encapsulated was acetic acid with relative concentration 47.50 %v/v and 19.76 %v/v respectively.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	encapsulation, maltodekstrin, Streptococcus lactis sp, organic acid, and antibacterial
Subjects:	Q Science > QD Chemistry > QD241-441 Organic chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Mrs Furika Furika
Date Deposited:	09 Mar 2022 06:34
Last Modified:	09 Mar 2022 06:34
URI:	http://repository.unsri.ac.id/id/eprint/65912

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