ELUSIDASI STRUKTUR DAN UJI AKTIVITAS ANTIOKSIDAN SENYAWA METABOLIT SEKUNDER DARI JAMUR ENDOFITIK Aspergillus Fumigatus PADA TANAMAN SAMBILOTO (Andrographis paniculata Nees)

SARI, RIA PUTRI INDAH and Elfita, Elfita and Muharni, Muharni (2012) ELUSIDASI STRUKTUR DAN UJI AKTIVITAS ANTIOKSIDAN SENYAWA METABOLIT SEKUNDER DARI JAMUR ENDOFITIK Aspergillus Fumigatus PADA TANAMAN SAMBILOTO (Andrographis paniculata Nees). Undergraduate thesis, Sriwijaya University.

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Abstract

Endofitik microbe is an organism in the form of bacteria or fungi that form colonies in plant tissues, but does not harm the host plant. In the sambiloto plant (Andrographis paniculata Nees) one secondary metabolites of fungi Aspergillus fumigatus endofitik has been isolated. Isolation begins with the cultivation of the fungus Aspergillus fumigatus in media 5L PDB (potato dextrose broth) for eight weeks. Media was extracted with ethyl acetate solvent and the extract obtained was concentrated by rotary evaporator. Ethyl acetate extracts were separated by chromatographic techniques to obtain pure compound. The molecular structure of the isolated compound was determined by analysis of NMR spectroscopic data, and antioxidant activity assay by l.l-diphenyl-2-pikrihidrazyil (DPPH) methode with variations concentration of 15.62, 31.25, 62.50, 125, 250, 500 and 1000 ppm. The isolated form white crystals. As data analysis of specstroscopy, known the isolated result is part of pyranon that 5-hydroxy pyranon-4-(hydroxymethyl)-2Hpyran-2-one with the molecular formula CgHgO^ The isolated compound showed antioxidant activity with IC50 value of 53.25 ppm.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Fungi endofitik, Aspergillus fumigatus, Andrographis paniculata Nees
Subjects:	Q Science > QD Chemistry > QD241-441 Organic chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Mrs Furika Furika
Date Deposited:	14 Mar 2022 03:11
Last Modified:	14 Mar 2022 03:11
URI:	http://repository.unsri.ac.id/id/eprint/66080

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