ISOLASI SENYAWA METABOLIT SEKUNDER DARI TANAMAN SAMBILOTO (Andographis paniculata Nees) DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP Escherichia coli DAN Shigella dysentriae

LESTARI, ZAIRAH and Elfita, Elfita and Miksusanti, Miksusanti (2012) ISOLASI SENYAWA METABOLIT SEKUNDER DARI TANAMAN SAMBILOTO (Andographis paniculata Nees) DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP Escherichia coli DAN Shigella dysentriae. Undergraduate thesis, Sriwijaya University.

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Abstract

A secondary metabolic compound was isolated fforn ethyl acetate fraction of sambiloto plant extract (Andrographis paniculata Ness). The extraction was done by maceration method. Separation and purification was counducted by chromatographic technique to obtained pure compounds of white crystal 55 mg. The structure of this compound was determined with UV, IR, NMR 1-D ('HNMR, 13C- NMR, and DEPT), 2-D (HMQC, HMBC, and COSY), and it’s antibacterial activity was tested by diffusion method using Escherichia coli and Shigella dysentriae as bacterial tested. Based on spectral data analysis could be concluded that pure compound that has been isolated was diterpenoid lactone Andrographolide (C20H30O5). The test results showed that the antibacterial activity of the extract at a concentration of 625 ppm viscous able to inhibit the growth of bacteria Escherichia coli and Shigella dysentriae inhibitory diameter 5 mm and 6 mm, whereas the pure compound has inhibitory diameter of 7 mm and 8 mm after reduction with paper discs. The pure compounds of andrographolide has more antibacterial activity than crude extract of sambiloto. The inhibition of amoxicillin with a diameter of 15 mm has more antibacterial activity than extracts and pure compounds to Shigella dysentriae and Escherichia coli than concentrations of 625 ppm.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Andrographis paniculata Ness, Andrographolide, Escherichia coli, Shigella dysentriae
Subjects:	Q Science > QD Chemistry > QD146-197 Inorganic chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Mrs Furika Furika
Date Deposited:	15 May 2022 07:11
Last Modified:	15 May 2022 07:11
URI:	http://repository.unsri.ac.id/id/eprint/69915

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