ISOLASI, KARAKTERISASI, DAN UJI AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER DARI MIKROBA ENDOFITIK Aspergillus fumigatus PADA KULIT BATANG KANDIS GAJAH (Garcinia griffithii T. Anders)

WAHYUNI, TRI INDAH and Elfita, Elfita and Muharni, Muharni (2012) ISOLASI, KARAKTERISASI, DAN UJI AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER DARI MIKROBA ENDOFITIK Aspergillus fumigatus PADA KULIT BATANG KANDIS GAJAH (Garcinia griffithii T. Anders). Undergraduate thesis, Sriwijaya University.

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Abstract

The secondary metabolite compound from Aspergillus fumigatus endophytic fungus on the stem bark of kandis gajah (Garcinia griffithii T Anders) has been isolated. The isolation was begun with the cultivation of the Aspergillus fumigatus fiingus in 5 L of media PDB (Potato Dextrose Broth) for 8 weeks. The medium was extracted with ethyl acetate and the extract which obtained was concentrated by rotary evaporator. Ethyl acetate extract was separated and purified by chromatographic techniques to obtain pure compounds. The molecular structure of the compound was determined base on spectroscopy data NMR 1D whereas antioxidant activity test by use DPPH (l,l-diphenyl-2-pikrilhidrazil) method at concentration 500, 250, 125, 62.5 and 31.25 ppm. Based on spectroscopic data analysis it could be concluded that the compound was aliphatic with 28 carbon atoms and 38 protons attached to the carbon atom. This compound gave IC50 value 500.79 ppm, and confirmed inactive as an antioxidant

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	endophytic fungus, Aspergillus fumigatus, Garcinia griffithii T anders.
Subjects:	Q Science > QD Chemistry > QD146-197 Inorganic chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Mrs Furika Furika
Date Deposited:	15 May 2022 07:26
Last Modified:	15 May 2022 07:26
URI:	http://repository.unsri.ac.id/id/eprint/69952

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