PENGARUH WAKTU SENTRIFUGASI PADA SEXING SPERMATOZOA DENGAN MEDIA BOVINE SERUM ALBUMIN TERHADAP MORFOLOGI DAN MEMBRAN PLASMA UTUH SPERMATOZOA X-Y SAPI SIMMENTAL

KHOTIMAH, KHUSNUL and Priyanto, Langgeng (2022) PENGARUH WAKTU SENTRIFUGASI PADA SEXING SPERMATOZOA DENGAN MEDIA BOVINE SERUM ALBUMIN TERHADAP MORFOLOGI DAN MEMBRAN PLASMA UTUH SPERMATOZOA X-Y SAPI SIMMENTAL. Undergraduate thesis, Sriwijaya University.

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Abstract

Sexing and centrifugation of spermatozoa is one way to improve the quality of spermatozoa. This study aimed to obtain the right centrifugation time for sexing spermatozoa with Bovine Serum Albumin media on the morphology and intact plasma membrane of X-Y spermatozoa in Simmental cattle. This research was carried out from October 2021 to November 2021 at the Laboratory of Animal Reproduction and Health at the Forage Breeding Center for Animal Feed, Sembawa. The design used in this study was a completely randomized design (CRD) with 3 treatments and 4 replications. Centrifugation treatment for sexing spermatozoa with BSA media P1: centrifugation time 4 minutes, P2: centrifugation time 8 minutes, P3: centrifugation time 12 minutes. The parameters observed were intact plasma membranes and morphology of spermatozoa X and Y. The results showed that the centrifugation time of sexing spermatozoa was different from that of Bovine Serum Albumin (BSA) media. Y spermatozoa abnormalities had a significant effect (P<0.05). The intact plasma membrane of spermatozoa in both X and Y spermatozoa had a significant effect (P<0.05) on the centrifugation time treatment. The results of using the right time are in the P1 treatment with a centrifugation time of 4 minutes where the value of the upper layer of intact plasma membrane X sexing cement is 62.93%, the lower layer Y is 63.94%, while the abnormality value in the upper X is 20 ,91% and the lower Y is 25,34%.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Bovine Serum Albumin, Centrifugation, Spermatozoa, X-Y.
Subjects:	S Agriculture > SF Animal culture > SF1-1100 Animal culture
Divisions:	05-Faculty of Agriculture > 54231-Animal Husbandry (Animal Science) (S1)
Depositing User:	Khusnul Khotimah
Date Deposited:	18 May 2022 08:24
Last Modified:	18 May 2022 08:24
URI:	http://repository.unsri.ac.id/id/eprint/70172

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